CHARACTERIZATION OF 8 MONOCLONAL-ANTIBODIES TO INVOLUCRIN
CHARACTERIZATION OF 8 MONOCLONAL-ANTIBODIES TO INVOLUCRIN
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DOI：
10.1089/hyb.1992.11.367
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发表时间：
1992-06-01
期刊：
影响因子：
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通讯作者：
WATT, FM
中科院分区：
文献类型：
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作者：
HUDSON, DL;WEILAND, KL;WATT, FM
外皮蛋白是不溶性蛋白质包膜的前体，其组装在表皮的最外层。该蛋白质的编码序列包含许多短串联重复序列，这些重复序列在哺乳动物进化过程中发生了很大变化。我们已经确定了8个小鼠单克隆抗体对人类外皮蛋白，所有这些结合的蛋白质的免疫沉淀，免疫印迹和免疫组织化学制剂。 每种抗体与大猩猩、猫头鹰猴、狗和猪外皮蛋白以及与人蛋白质片段的交叉反应性进行筛选，所述人蛋白质片段表达于p53-gt 11中，其包括现代重复片段的整个早期区域。 三种抗体在所有这些测定中识别外皮蛋白。四种抗体识别灵长类动物的外皮蛋白和GT 11片段。 一种抗体，这表明与低分子量的蛋白质的交叉反应，只承认灵长类动物involucrins，因此绑定的早期区域以外的现代部分。 由于抗体可用于检测involucrin的生化和组织学，在一系列的物种，他们将有进一步的研究中的蛋白质的表达，功能和进化的应用。
Involucrin is a precursor of the insoluble protein envelope that is assembled in the outermost layers of the epidermis. The coding sequence of the protein contains a number of short tandem repeals that have been greatly altered during mammalian evolution. We have characterised eight mouse monoclonal antibodies raised against human involucrin, all of which bind to the protein in immunoprecipitation, immunoblot and immunohistochemical preparations. Each antibody was screened for cross-reactivity with gorilla, owl monkey, dog and pig involucrin and with a fragment of the human protein, expressed in lambda-gt 11, that includes the entire early region of the modern segment of repeals. Three antibodies recognised involucrin in all of these assays. Four antibodies recognised primate involucrins and the lambda-gt 11 fragment. One antibody, which showed cross-reactivity with lower molecular weight proteins, only recognised primate involucrins and therefore bound outside the early region of the modern segment. Since the antibodies can be used to detect involucrin both biochemically and histologically, in a range of species, they will have applications in further studies of the expression, function and evolution of the protein.