ERYTHROPOIETIN HAS A MITOGENIC AND POSITIVE CHEMOTACTIC EFFECT ON ENDOTHELIAL-CELLS
ERYTHROPOIETIN HAS A MITOGENIC AND POSITIVE CHEMOTACTIC EFFECT ON ENDOTHELIAL-CELLS
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DOI：
10.1073/pnas.87.15.5978
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发表时间：
1990-08-01
影响因子：
11.1
通讯作者：
STEINER, M
中科院分区：
文献类型：
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作者：
ANAGNOSTOU, A;LEE, ES;STEINER, M
促红细胞生成素是一种造血生长因子，对红系祖细胞的增殖和分化具有特异性作用。我们观察到人重组促红细胞生成素对人脐静脉内皮细胞和牛肾上腺毛细血管内皮细胞的增殖作用具有剂量依赖性。用放射性碘标记的重组人促红细胞生成素的结合研究揭示了大量的（约27，000）的亲和力在10-9 M范围内的明显单一类别的受体。通过双功能交联剂的放射性标记的配体与其受体的连接使我们能够鉴定45 kDa的内皮细胞蛋白作为与促红细胞生成素的促有丝分裂作用相关的主要受体。重组人血管内皮素还能促进内皮细胞的迁移。
Ertyropoietin is known to be a hematopoietic growth factor with a singularly specific action on the proliferation and differentiation of erythroid progenitor cells. We have observed a dose-dependent proliferative action of human recombinant erythropoietin on human umbilical vein endothelial cells and bovine adrenal capillary endothelial cells. Binding studies with radioiodinated recombinant human erythropoietin revealed a large number (.apprxeq. 27,000) of an apparent single class of receptors with an affinity in the 10-9 M range. Linkage of the radiolabeled ligand to its receptor via a bifunctional crosslinking agent allowed us to identify an endothelial cell protein of 45 kDa as the principal receptor associated with this mitogenic effect of erythropoietin. Recombinant human erythtopoietin also enhanced the migration of endothelial cells.