The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae.
The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae.
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溶肉菌（一种小变形虫的专性细胞内细菌寄生虫）的系统发育状态。
DOI：
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发表时间：
1992
影响因子：
2.1
通讯作者：
K. Schleifer
中科院分区：
文献类型：
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作者：
N. Springer;W. Ludwig;W. Drozanski;R. Amann;K. Schleifer
利用聚合酶链反应结合位点特异性引物扩增了专性胞内寄生菌溶肉菌（Sarcobium lyticum）的16S rRNA基因。将扩增的DNA克隆、测序并与其他细菌16S rRNA序列进行比较。分析表明，S.溶菌属属于变形菌纲的γ亚类，并且显示出与通过从肺炎患者的痰中富集变形虫而回收的细胞内军团菌属物种最密切的关系。S.用荧光寡核苷酸探针通过全细胞杂交可原位检测溶胞菌。
A 16S rRNA gene of the obligate intracellular bacterial parasite Sarcobium lyticum was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was cloned, sequenced and compared with other bacterial 16S rRNA sequences. The analysis revealed that S. lyticum belongs to the gamma subclass of the Proteobacteria and shows the closest relationship to an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. S. lyticum could be detected in situ with a fluorescent oligonucleotide probe by whole cell hybridization.
影响因子：
56.9
作者：
DELONG, EF;WICKHAM, GS;PACE, NR
通讯作者：
PACE, NR