Multilocus enzyme electrophoresis and cytochrome B gene sequencing-based identification of Leishmania isolates from different foci of cutaneous leishmaniasis in Pakistan
Multilocus enzyme electrophoresis and cytochrome B gene sequencing-based identification of Leishmania isolates from different foci of cutaneous leishmaniasis in Pakistan
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DOI：
10.4269/ajtmh.2006.75.261
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发表时间：
2006-08-01
影响因子：
3.3
通讯作者：
Hashiguchi, Yoshihisa
中科院分区：
文献类型：
--
作者：
Marco, Jorge D.;Bhutto, Abdul M.;Hashiguchi, Yoshihisa
对从巴基斯坦患者皮损中分离的17株利什曼原虫进行了多位点酶电泳法和细胞色素b(Cytb)基因的聚合酶链式反应扩增及测序。11个表达9种酵母菌的群体被归入主要利什曼原虫。它们均分别从信德省和俾路支省拉卡纳区和锡比市的低地患者中分离出来。从俾路支省奎达市高地分离到的6株菌，分布在2个酵母菌群中(5个和1个)，鉴定为L.(L.)特罗皮卡。在物种水平上，对所有被检测的种群进行细胞色素b测序，得到了相同的结果。临床特征(湿型或干型皮损)与所涉及的利什曼原虫种类之间没有明确的关联。利什曼原虫Leishmania(L.)与L.(L.)特罗皮卡。这种差异可能是因为人类可能是L.(L.)然而，许多野生哺乳动物可能是L.(L.)主要研究人畜共患病周期。
Seventeen Leishmania stocks isolated from cutaneous lesions of Pakistani patients were studied by multilocus enzyme electrophoresis and by polymerase chain reaction amplification and sequencing of the cytochrome b (Cyt b) gene. Eleven stocks that expressed nine zymodemes were assigned to L. (Leishmania) major. All of them were isolated from patients in the lowlands of Larkana district and Sibi city in Sindh and Balochistan provinces, respectively. The remaining six, distributed in two zymodemes (five and one), isolated from the highland of Quetta city, Balochistan, were identified as L. (L.) tropica. The same result at species level was obtained by the Cyt b sequencing for all the stocks examined. No clear-cut association between the clinical features (wet or dry type lesions) and the Leishmania species involved was found. Leishmania (L.) major was highly polymorphic compared with L. (L.) tropica. This difference may be explained by the fact that humans may act as a sole reservoir of L. (L.) tropica in anthroponotic cycles; however, many wild mammals can be reservoirs of L. (L.) major in zoonotic cycles.