Stability of karyotype in serial isolates of Candida albicans from neutropenic patients.
Stability of karyotype in serial isolates of Candida albicans from neutropenic patients.
复制标题
中性粒细胞减少症患者白色念珠菌系列分离株核型的稳定性。
DOI：
10.1128/jcm.33.4.794-796.1995
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发表时间：
1995
影响因子：
9.4
通讯作者：
Scherer,S
中科院分区：
文献类型：
--
作者：
Barton,RC;vanBelkum,A;Scherer,S
从 20 名白血病患者长达 8 个月的时间里连续分离出白色念珠菌。之前已经描述了通过重复 PCR 和随机扩增多态性 DNA PCR 对这些分离株进行指纹分析 (A. van Belkum, W. Melchers, B. E. de Pauw, S. Scherer, W. Quint, and J. F. Meis, J. Infect. Dis. 169:1062-1070, 1994)。使用轮廓钳均质电场凝胶电泳来检查这些分离株的染色体。当在一系列分离株中观察到核型变化时，使用 27A 家族的重复 DNA 序列进行额外的重复 PCR 和 Southern 印迹分析。这两种基因分型工具用于确定一系列分离株中观察到的核型变化是否是由于单个菌株中的染色体重排或由于多个菌株的定植所致。确定一系列菌株中核型的变化表明被新菌株感染。
Serial isolates of Candida albicans were obtained from 20 patients with leukemia over periods of up to 8 months. The fingerprinting of these isolates by interrepeat PCR and random amplified polymorphic DNA PCR has been described previously (A. van Belkum, W. Melchers, B. E. de Pauw, S. Scherer, W. Quint, and J. F. Meis, J. Infect. Dis. 169:1062-1070, 1994). Contour-clamped homogeneous electric field gel electrophoresis was used to examine the chromosomes of these isolates. When changes in the karyotype were seen in a series of isolates, additional interrepeat PCR and Southern blotting with a repeat DNA sequence from the 27A family were performed. These two genotyping tools were used to determine if karyotypic changes seen in a series of isolates were due to chromosome rearrangements in a single strain or due to colonization with more than one strain. It was determined that changes in karyotype in a series of strains indicated infection by a new strain.