Temporal endurance of exercise-induced benefits on hippocampus-dependent memory and synaptic plasticity in female mice.
Temporal endurance of exercise-induced benefits on hippocampus-dependent memory and synaptic plasticity in female mice.
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DOI：
10.1016/j.nlm.2022.107658
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发表时间：
2022-10
影响因子：
2.7
通讯作者：
Keiser, A A
中科院分区：
文献类型：
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作者：
Dong, T N;Kramar, E A;Beardwood, J H;Al-Shammari, A;Wood, M A;Keiser, A A
运动促进海马神经发生和神经可塑性，从而促进认知功能。我们以前的研究已经证明，在雄性小鼠中，自愿运动能够在久坐动物长期记忆形成的亚阈值条件下实现海马区依赖性学习。这种认知增强可以在运动停止后很长时间内保持，并且可以通过随后的阈下运动会话重新参与，这表明运动引起的益处是时间动态的。在女性中，运动的益处可以维持的程度以及这种维持的机制尚未确定。在这里，我们检查了启动和维持雌性C57 BL/6 J小鼠运动益处所需的运动参数。使用阈下版本的视皮层依赖的任务，称为对象位置记忆（OLM）的任务，我们表明，14天的自愿运动，使学习阈下收购条件下的雌性小鼠。初始运动后，7天的久坐延迟导致性能下降，当动物在久坐延迟后接受2天的重新激活运动时，可以重新促进性能下降。发情周期的评估表明，增强车轮运行活动在发情期阶段相对于间情期，而发情期的培训或测试没有影响OLM性能。利用相同的运动参数，我们证明了14天的运动增强了海马CA 1区的长时程增强（LTP），这种效应在久坐延迟和恢复运动后持续存在。先前的研究已经提出运动诱导的BDNF上调作为运动介导的突触可塑性和认知益处的潜在机制。然而，我们的评估海马BDNF mRNA表达的记忆检索显示，运动条件和控制之间没有差异，这表明，持续的BDNF上调可能不需要维持运动诱导的好处。总之，我们的数据表明，14天的自愿运动可以对雌性小鼠的神经可塑性和认知功能产生持久的益处，这是第一个关于女性运动诱导益处的时间耐受性的证据。
Exercise facilitates hippocampal neurogenesis and neuroplasticity that in turn, promotes cognitive function. Our previous studies have demonstrated that in male mice, voluntary exercise enables hippocampus-dependent learning in conditions that are normally subthreshold for long-term memory formation in sedentary animals. Such cognitive enhancement can be maintained long after exercise has ceased and can be re-engaged by a subsequent subthreshold exercise session, suggesting exercise-induced benefits are temporally dynamic. In females, the extent to which the benefits of exercise can be maintained and the mechanisms underlying this maintenance have yet to be defined. Here, we examined the exercise parameters required to initiate and maintain the benefits of exercise in female C57BL/6J mice. Using a subthreshold version of the hippocampus-dependent task called object-location memory (OLM) task, we show that 14d of voluntary exercise enables learning under subthreshold acquisition conditions in female mice. Following the initial exercise, a 7d sedentary delay results in diminished performance, which can be re-facilitated when animals receive 2d of reactivating exercise following the sedentary delay. Assessment of estrous cycle reveals enhanced wheel running activity during the estrus phase relative to the diestrus phase, whereas estrous phase on training or test had no effect on OLM performance. Utilizing the same exercise parameters, we demonstrate that 14d of exercise enhances long-term potentiation (LTP) in the CA1 region of the hippocampus, an effect that persists throughout the sedentary delay and following the reactivating exercise session. Previous studies have proposed exercise-induced BDNF upregulation as the mechanism underlying exercise-mediated benefits on synaptic plasticity and cognition. However, our assessment of hippocampal Bdnf mRNA expression following memory retrieval reveals no difference between exercise conditions and control, suggesting that persistent Bdnf upregulation may not be required for maintenance of exercise-induced benefits. Together, our data indicate that 14d of voluntary exercise can initiate long-lasting benefits on neuroplasticity and cognitive function in female mice, establishing the first evidence on the temporal endurance of exercise-induced benefits in females.