AtRAV1 and AtRAV2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering
AtRAV1 and AtRAV2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering
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DOI：
10.1016/j.plantsci.2015.09.013
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发表时间：
2015-12-01
期刊：
影响因子：
5.2
通讯作者：
Rock, Christopher D.
中科院分区：
文献类型：
--
作者：
Mittal, Amandeep;Jiang, Yingwen;Rock, Christopher D.
棉纤维品质与高产量之间存在反比关系，这是一个长期存在的问题。为了更好地了解棉花（Gossypium hirsutum）纤维发育中的干旱胁迫信号和适应，我们表达了拟南芥转录因子RELATED_TO_ABA_INSENSITIVE3/VIVIPAROUS1/(RAV1)和AtRAV2，它们编码APETALA2-Basic3结构域蛋白，显示可抑制FLOWERING_LOCUS_T(FT)的转录并促进气孔开放 细胞自主地。在三年的田间试验中，我们表明，AtRAV1 和 AtRAV2 过表达棉花的纤维显着延长了约 5%，但在浇水良好或干旱胁迫条件下，产量仅略有下降，导致对照植物产量损失 40-60%，纤维长度损失 3-7%。来自干旱胁迫转基因的较长转基因纤维可以纺成纱线，该纱线比来自充分浇水的对照纤维的纱线明显更坚固且更均匀。转基因 AtRAV1 和 AtRAV2 品系开花较晚，并在较高节处保留棉铃，这与内源 GhFT-Like (FTL) 转录物积累的抑制相关。在胚珠发育早期，观察到参与纤维起始的 GhRAV2L、GhMYB25-Like (MYB25L) 以及参与纤维伸长的 GhMYB2 和 GhMYB25 的表达升高。控制发育和环境信号层次中关键节点的 RAV 表达的改变具有作物表型修饰的潜力。 (C) 2015 Elsevier Ireland Ltd. 保留所有权利。
There is a longstanding problem of an inverse relationship between cotton fiber qualities versus high yields. To better understand drought stress signaling and adaptation in cotton (Gossypium hirsutum) fiber development, we expressed the Arabidopsis transcription factors RELATED_TO_ABA_INSENSITIVE3/VIVIPAROUS1/(RAV1) and AtRAV2, which encode APETALA2-Basic3 domain proteins shown to repress transcription of FLOWERING_LOCUS_T(FT) and to promote stomatal opening cell-autonomously. In three years of field trials, we show that AtRAV1 and AtRAV2-overexpressing cotton had similar to 5% significantly longer fibers with only marginal decreases in yields under well-watered or drought stress conditions that resulted in 40-60% yield penalties and 3-7% fiber length penalties in control plants. The longer transgenic fibers from drought-stressed transgenics could be spun into yarn which was measurably stronger and more uniform than that from well-watered control fibers. The transgenic AtRAV1 and AtRAV2 lines flowered later and retained bolls at higher nodes, which correlated with repression of endogenous GhFT-Like (FTL) transcript accumulation. Elevated expression early in development of ovules was observed for GhRAV2L, GhMYB25-Like (MYB25L) involved in fiber initiation, and GhMYB2 and GhMYB25 involved in fiber elongation. Altered expression of RAVs controlling critical nodes in developmental and environmental signaling hierarchies has the potential for phenotypic modification of crops. (C) 2015 Elsevier Ireland Ltd. All rights reserved.