DISSECTING THE CENTROMERE OF THE HUMAN Y-CHROMOSOME WITH CLONED TELOMERIC DNA
DISSECTING THE CENTROMERE OF THE HUMAN Y-CHROMOSOME WITH CLONED TELOMERIC DNA
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DOI：
10.1093/hmg/3.8.1227
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发表时间：
1994-08-01
影响因子：
3.5
通讯作者：
BROWN, WRA
中科院分区：
文献类型：
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作者：
BROWN, KE;BARNETT, MA;BROWN, WRA
我们使用端粒DNA打断了阿尔法卫星DNA着丝粒阵列中的人类Y染色体，并创建了两条衍生染色体;一条由短臂和140 kb的阿尔法DNA组成，另一条由长臂和480 kb的阿尔法DNA组成。在有丝分裂时准确分离。已知在α DNA周围没有大规模的序列重复，因此对我们的结果的最简单解释是负责精确分离的序列是α DNA本身。虽然长臂近端着丝粒衍生物准确分离，但在约10%的后期细胞中，它相对于其他染色体滞后，因此可能需要额外的序列来进行有序分离。短臂近端着丝粒染色体的大小可能不超过12 Mb，因此我们的结果也表明，这种大小的染色体能够准确分离。
We have used telomeric DNA to break the human Y chromosome within the centromeric array of alphoid satellite DNA and have created two derivative chromosomes; one consists of the short arm and 140 kb of alphoid DNA, the other consists of the long arm and 480 kb of alphoid DNA. Bath segregate accurately at mitosis. It is known that there is no large scale sequence duplication around the alphoid DNA and so the simplest interpretation of our results is that the sequence responsible for accurate segregation is the alphoid DNA itself. Although the long arm acrocentric derivative segregates accurately it lags with respect to the other chromosomes in about 10% of anaphase cells and thus additional sequences may be required for orderly segregation. The short arm acrocentric chromosome is probably no larger than 12 Mb in size and thus our results also demonstrate that chromosomes of this size are capable of accurate segregation.