Effect of bFGF on invasion of ovarian cancer cells through the regulation of Ets-1 and urokinase-type plasminogen activator
Effect of bFGF on invasion of ovarian cancer cells through the regulation of Ets-1 and urokinase-type plasminogen activator
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DOI：
10.3109/13880200903062630
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发表时间：
2010-02-01
影响因子：
3.8
通讯作者：
Jiang, Sen
中科院分区：
文献类型：
--
作者：
Li, Ting;Jiang, Sen
本研究的目的是探讨碱性成纤维细胞生长因子（bFGF）在卵巢癌进展中的作用。通过研究bFGF对SKOV（3）卵巢癌细胞尿激酶型纤溶酶原激活物（uPA）分泌和肿瘤细胞侵袭的影响来完成。体外培养人卵巢癌细胞株SKOV（3）。诱导SKOV（3）细胞表达uPA基因和蛋白，RT-PCR检测bFGF对SKOV（3）细胞uPA基因表达的影响，ELISA检测bFGF对uPA蛋白表达的影响。采用脂质体法将Ets-1反义寡核苷酸转染SKOV（3）细胞。观察bFGF对SKOV（3）细胞Ets-1表达及侵袭能力的影响。SKOV（3）细胞中uPA基因和蛋白的表达均被诱导，p < 0.05。Ets-1反义寡核苷酸能抑制SKOV（3）细胞uPA的表达，p < 0.05。Ets-1反义寡核苷酸可使SKOV（3）细胞的侵袭能力增强2.3倍，并可抑制Ets-1反义寡核苷酸的侵袭能力。bFGF可通过诱导uPA的表达增强卵巢癌细胞的体外侵袭能力，这种作用也受到转录因子Ets-1的调控。
The aim of the study was to explore the role of basic fibroblast growth factor (bFGF) in ovarian cancer progression. This was done by investigating the effects of bFGF on both the secretion of urokinase-type plasminogen activator (uPA) and the invasion of tumor cells in SKOV(3) ovarian cancer cells. Human ovarian cancer cell line SKOV(3) was cultured in vitro. The expression of uPA gene and protein was induced in SKOV(3) cells; the impact of bFGF on the expression of uPA gene in SKOV(3) cells was studied by RT-PCR, and the impact of bFGF on the expression of uPA protein was tested by ELISA. Ets-1 antisense oligonucleotides were transfected into SKOV(3) cells by liposome protocol. The effects of bFGF on Ets-1 expression and the invasion ability of SKOV(3) cells were determined both before and after exposure to different concentrations of bFGF for 24 h. The expression of both uPA gene and protein was induced in SKOV(3) cells, p < 0.05. The expression of uPA was suppressed by Ets-1 antisense oligonucleotides in SKOV(3) cells, p < 0.05. The invasion ability of SKOV(3) cells was increased by 2.3-fold, and this effect was also suppressed by Ets-1 antisense oligonucleotides. bFGF can enhance the invasion ability of ovarian cancer cells in vitro by inducing the expression of uPA, and this effect is also regulated by the transcription factor Ets-1.