Arsenic Methylation in Arabidopsis thaliana Expressing an Algal Arsenite Methyltransferase Gene Increases Arsenic Phytotoxicity.
Arsenic Methylation in Arabidopsis thaliana Expressing an Algal Arsenite Methyltransferase Gene Increases Arsenic Phytotoxicity.
复制标题
表达藻类亚砷酸甲基转移酶基因的拟南芥中的砷甲基化增加了砷的植物毒性
DOI：
10.1021/acs.jafc.6b00462
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发表时间：
2016-04-06
影响因子：
6.1
通讯作者：
Zhao FJ
中科院分区：
文献类型：
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作者：
Tang Z;Lv Y;Chen F;Zhang W;Rosen BP;Zhao FJ
土壤中的砷污染可导致砷向食物链转移的增加。一种潜在的缓解策略是对植物进行基因工程改造，使它们能够将无机砷转化为甲基化和挥发性砷。本研究利用真核藻类莱茵衣藻（Chlamydomonas reinhardtii）的亚砷酸盐（As(III)） s -腺苷甲基转移酶（arsM）基因对拟南芥两种生态型进行了基因工程改造。转基因拟蓝获得了较强的砷甲基化能力，在茎部将大部分无机砷转化为二甲基砷酸盐[DMA(V)]。从转基因植株中检测到少量挥发性砷。然而，转基因植株在培养基中对As（III）更加敏感，表明DMA(V)比无机As更具植物毒性。该研究证明了工程As甲基化在植物中的负面影响，并指出需要具有强甲基化As能力的arsM基因。
Arsenic (As) contamination in soil can lead to elevated transfer of As to the food chain. One potential mitigation strategy is to genetically engineer plants to enable them to transform inorganic As to methylated and volatile As species. In this study, we genetically engineered two ecotypes of Arabidopsis thaliana with the arsenite (As(III)) S-adenosylmethyltransferase (arsM) gene from the eukaryotic alga Chlamydomonas reinhardtii. The transgenic A. thaliana plants gained a strong ability to methylate As, converting most of the inorganic As into dimethylarsenate [DMA(V)] in the shoots. Small amounts of volatile As were detected from the transgenic plants. However, the transgenic plants became more sensitive to As(III) in the medium, suggesting that DMA(V) is more phytotoxic than inorganic As. The study demonstrates a negative consequence of engineered As methylation in plants and points to a need for arsM genes with a strong ability to methylate As to volatile species.