EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA-1 IN SPECIFIC CELLS AND TISSUES OF ADULT AND NEONATAL MICE
EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA-1 IN SPECIFIC CELLS AND TISSUES OF ADULT AND NEONATAL MICE
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DOI：
10.1083/jcb.108.2.661
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发表时间：
1989-02-01
影响因子：
7.8
通讯作者：
SPORN, MB
中科院分区：
文献类型：
--
作者：
THOMPSON, NL;FLANDERS, KC;SPORN, MB
我们已经使用免疫组织化学技术来检测成年和新生小鼠的许多组织中的转化生长因子-β 1（TGF-1）。针对TGF-β 1的氨基末端30个氨基酸产生的两种抗体中的每一种在细胞内或细胞外位置选择性地染色该分子。在肾上腺皮质、骨髓巨核细胞和其他细胞、心肌细胞、软骨细胞、肾远端小管、卵巢腺细胞和胎盘绒毛膜细胞中发现了强细胞内染色。在软骨、心脏、胰腺、胎盘、皮肤和子宫中发现细胞外基质的显著染色。染色通常在给定组织的特化细胞中特别强烈，表明TGF-β的独特作用。在那个组织里。TGF-β 1的mRNA表达水平及其组织化学染色在给定组织中不一定相关，如在脾中。目前的数据进一步支持TGF-β抑制剂的概念。在控制上皮细胞和周围间质之间的相互作用中具有重要作用。
We have used immunohistochemical techniques to detect transforming growth factor-.beta.1 (TGF-1) in many tissues of adult and neonatal mice. Each of two antibodies raised to the amino-terminal 30 amino acids of TGF-.beta.1 selectively stained this molecule in either intracellular or extracellular locations. Strong intracellular staining was found in adrenal cortex, megakaryocytes and other cells of the bone marrow, cardiac myoctes, chondrocytes, renal distal tubules, ovarian glandular cells, and chorionic cells of the placenta. Marked staining of extracellular matrix was found in cartilage, heart, pancreas, placenta, skin, and uterus. Staining was often particularly intense in specialized cells of a given tissue, suggesting unique roles for TGF-.beta. within that tissue. Levels of expression of mRNA for TGF-.beta.1 and its histochemical staining did not necessarily correlate in a given tissue, as in the spleen. The present data lend further support to the concept that TGF-.beta. has an important role in controlling interactions between epithelia and surrounding mesenchyme.