The effect of gradual or acute arterial occlusion on skeletal muscle blood flow, artcriogenesis, and inflammation in rat hindlimb ischemia
The effect of gradual or acute arterial occlusion on skeletal muscle blood flow, artcriogenesis, and inflammation in rat hindlimb ischemia
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DOI：
10.1016/j.jvs.2004.11.012
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发表时间：
2005-02-01
影响因子：
4.3
通讯作者：
Messina, LM
中科院分区：
文献类型：
--
作者：
Tang, GL;Chang, DS;Messina, LM
背景：目前严重肢体缺血的实验模型是基于急性缺血而不是慢性缺血。人类外周血管疾病主要是慢性缺血的结果。我们假设慢性后肢缺血模型比急性后肢缺血模型会产生更多的侧支动脉，更多的血流量，更少的坏死和炎症。因此，我们建立了一种大鼠慢性后肢缺血模型，并比较了慢性缺血和急性缺血对后肢骨骼肌的影响。在36只雄性Sprague-Dawley大鼠中诱导急性或慢性缺血。慢性缺血造成的血流量，通过激光多普勒扫描和肌肉氧张力测量证实，逐渐减少超过1至2周后operation.Results：组织学分析显示，慢性后肢缺血更好地保存肌肉质量和架构，刺激毛细血管新生，而缺乏急性缺血后看到的肌肉坏死和炎性细胞浸润。令人惊讶的是，通过激光多普勒测量，慢性缺血组比急性缺血组恢复真皮血流更慢，更不完全（0.66 +/- 0.02 vs 0.76 +/- 0.04，P
Background: Current experimental models of critical limb ischemia are based on acute ischemia rather than on chronic ischemia. Human peripheral vascular disease is largely a result of chromic ischemia. We hypothesized that a model of chronic hindlimb ischemia would develop more collateral arteries, more blood flow, and less necrosis and inflammation than would acute hindlimb ischemia. We therefore developed a rat model of chronic hindlimb ischemia and compared the effects of chronic ischemia with those of acute ischemia on hindlimb skeletal muscle.Methods. Acute or chronic ischemia was induced in 36 male Sprague-Dawley rats. Chronic ischemia caused blood flow, as measured by laser Doppler scanning and confirmed by muscle oxygen tension measurements, to gradually decrease over I to 2 weeks after operation.Results: Histologic analysis showed chronic hindlimb ischemia better preserved muscle mass and architecture and stimulated capillary angiogenesis, while lacking the muscle necrosis and inflammatory cell infiltrate seen after acute ischemia. Surprisingly, the chronic ischemia group recovered dermal blood flow more slowly and less completely than did the acute ischemia group, as measured by laser Doppler (0.66 +/- 0.02 vs 0.76 +/- 0.04, P