Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi.
Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi.
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荔枝果实脱落过程中组蛋白修饰物的全基因组鉴定及其表达模式
DOI：
10.3389/fpls.2017.00639
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发表时间：
2017
影响因子：
5.6
通讯作者：
Zhao M
中科院分区：
文献类型：
--
作者：
Peng M;Ying P;Liu X;Li C;Xia R;Li J;Zhao M
组蛋白的修饰，包括乙酰化和甲基化过程，在植物发育以及胁迫反应中的基因表达调控中起着至关重要的作用。然而，有限的信息催化组蛋白乙酰化和甲基化的非模式植物中的酶是目前可用的。本研究从荔枝（Litchi chinensis Sonn.）中鉴定了几种组蛋白修饰物（HM），包括6种组蛋白乙酰转移酶（HAT）、11种组蛋白脱乙酰酶（HDAC）、48种组蛋白甲基转移酶（HMT）和22种组蛋白脱甲基酶（HDM）。CV. Feizixiao）基于其序列与拟南芥（A. thaliana）、番茄（Solanum lycopersicum）和水稻（Oryza sativa）。系统发育分析表明，HM酶可以分为4个HAT，两个HDAC，两个HMT，和两个HDM亚家族，分别，而进一步的表达谱分析表明，17 HM在两个领域的处理果实脱落过程中显着改变。分析表明，这些基因表现出四种不同的模式的表达，在响应果实成熟，而在体外测定被用来确认HDAC活性的LcHDA2，LcHDA6，和LcSRT 2。我们的研究结果是第一次深入分析荔枝基因组中的HMs，并暗示一些可能在这种商业重要植物的果实脱落中发挥重要作用。
Modifications to histones, including acetylation and methylation processes, play crucial roles in the regulation of gene expression in plant development as well as in stress responses. However, limited information on the enzymes catalyzing histone acetylation and methylation in non-model plants is currently available. In this study, several histone modifier (HM) types, including six histone acetyltransferases (HATs), 11 histone deacetylases (HDACs), 48 histone methyltransferases (HMTs), and 22 histone demethylases (HDMs), are identified in litchi (Litchi chinensis Sonn. cv. Feizixiao) based on similarities in their sequences to homologs in Arabidopsis (A. thaliana), tomato (Solanum lycopersicum), and rice (Oryza sativa). Phylogenetic analyses reveal that HM enzymes can be grouped into four HAT, two HDAC, two HMT, and two HDM subfamilies, respectively, while further expression profile analyses demonstrate that 17 HMs were significantly altered during fruit abscission in two field treatments. Analyses reveal that these genes exhibit four distinct patterns of expression in response to fruit abscission, while an in vitro assay was used to confirm the HDAC activity of LcHDA2, LcHDA6, and LcSRT2. Our findings are the first in-depth analysis of HMs in the litchi genome, and imply that some are likely to play important roles in fruit abscission in this commercially important plant.