Structural study of the sugar chains of alpha-amylases produced ectopically in tumors.
Structural study of the sugar chains of alpha-amylases produced ectopically in tumors.
复制标题
肿瘤异位产生的 α-淀粉酶糖链的结构研究。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
A. Kobata
中科院分区：
文献类型：
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作者：
K. Yamashita;Y. Tachibana;T. Takeuchi;A. Kobata
从卵巢浆液性乳头状囊腺癌（病例1）和肺细支气管肺泡腺癌（病例2）产生的两种α-淀粉酶中，约30%分别是含有1 mol天冬酰胺连接糖链的糖蛋白。通过连续酶促降解和甲基化分析发现糖部分的结构如下：[（Gal β 1导致4）0或1GlcNAc β 1导致2 Man α 1导致6（3）][GlcNAc β 1导致2 Man α 1导致3（6）]Man β 1导致4GlcNAc β 1导致4（Fuc α 1导致6）GlcNAc和[（Gal β 1导致4）0或1GlcNAc β 1导致2 Man α 1导致6][NeuAc α 2导致6 Gal β 1导致4GlcNAc β 1导致2 Man α 1导致3]Man β 1导致4GlcNAc β 1导致4（Fuc α 1导致6）GlcNAc。例1和例2肿瘤中天冬酰胺连接的糖链的结构相同，与腮腺淀粉酶的结构相比是不完整的。
About thirty percent of two alpha-amylases produced from a serous papillary cystadenocarcinoma of the ovarium (case 1) and a bronchioloalveolar adenocarcinoma of the lung (case 2) was glycoproteins containing 1 mol of asparagine-linked sugar chain, respectively. The structures of the sugar moieties were found by sequential enzymatic degradation and methylation analysis to be as follows: [(Gal beta 1 leads to 4)0 or 1GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(3)][GlcNAc beta 1 leads to 2Man alpha 1 leads to 3(6)]Man beta 1 leads to 4GlcNAc beta 1 leads to 4(Fuc alpha 1 leads to 6)GlcNAc and [(Gal beta 1 leads to 4)0 or 1GlcNAc beta 1 leads to 2Man alpha 1 leads to 6][NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]Man beta 1 leads to 4GlcNAc beta 1 leads to 4(Fuc alpha 1 leads to 6)GlcNAc. Structures of asparagine-linked sugar chains were the same in the tumors of cases 1 and 2 and were incomplete in comparison with those of the parotid amylase.