Genetic characterization of parthenogenic Fasciola sp in Japan on the basis of the sequences of ribosomal and mitochondrial DNA
Genetic characterization of parthenogenic Fasciola sp in Japan on the basis of the sequences of ribosomal and mitochondrial DNA
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DOI：
10.1017/s0031182005008292
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发表时间：
2005-11-01
期刊：
影响因子：
2.4
通讯作者：
Fukuda, K
中科院分区：
文献类型：
--
作者：
Itagaki, T;Kikawa, A;Fukuda, K
在日本准确鉴定无精子片形吸虫类型仍然很困难，因为它们的形态变化。为了从遗传学角度对该虫型进行鉴定，对日本16个都道府县34只肝吸虫的核糖体内转录间隔区（ITS 1和ITS 2）、线粒体细胞色素c氧化酶I（COI）和NADH脱氢酶I（ND I）基因的核苷酸序列进行了分析。以Fsp 1和Fsp 2为代表的两种主要形式的序列与F. hepatica和F.在所有4个DNA标记中，日本的主要分布区分别是日本的北方和东西部。Fsp 1和Fsp 2可能是通过朝鲜半岛与2个不同谱系的感染牛一起传入日本的，并随着每个牛谱系的移动在日本的有限地区（北方和东西部）传播。日本型（Fsp 1/2）在核糖体DNA上表现为杂合性，在线粒体DNA上表现为Fsp 2单倍型，可能起源于父本F. hepatica和母体F.南极洲
Accurate identification of aspermic Fasciola forms in Japan remains difficult because of their morphological variations. In order to characterize the forms genetically, nucleotide sequences of ribosomal internal transcribed spacer (ITS1 and ITS2) and mitochondrial cytochrome c oxidase I (COI) and NADH dehydrogenase I (ND I) genes in 34 liver flukes from 16 prefectures in Japan were analysed. Two major forms represented by Fsp 1 and Fsp 2 had sequences identical to or closely resembling those of F. hepatica and F. gigantica, respectively, in all the 4 DNA markers and were mainly distributed in northern and eastern-western parts of Japan, respectively. Fsp 1 and Fsp 2 would have been introduced into Japan with infected cattle of 2 distinct lineages via the Korean Peninsula and spread through limited parts of Japan (northern and eastern-western parts) together with the movement of each cattle lineage. The Japanese form (Fsp 1/2), which showed heterozygosity in ribosomal DNA and Fsp 2 haplotype in mitochondrial DNA, may have originated in interspecific cross hybridization between paternal F. hepatica and maternal F. gigantica.