Preferential interaction of the core histone tail domains with linker DNA
Preferential interaction of the core histone tail domains with linker DNA
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DOI：
10.1073/pnas.121171498
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发表时间：
2001-06-05
影响因子：
11.1
通讯作者：
Hayes, JJ
中科院分区：
文献类型：
--
作者：
Angelov, D;Vitolo, JM;Hayes, JJ
在染色质内，核心组蛋白尾部结构域在调节染色质纤维内的核小体DNA的结构和可及性中起关键作用。因此，许多核过程是由这些结构域的伴随翻译后修饰促进的。然而，阐明的机制，尾巴介导这样的过程中等待染色质内的尾巴相互作用的定义，在这项研究中，我们已经调查了主要的DNA靶的大多数的尾巴在单核细胞。结果清楚地表明，尾部优先结合“接头”DNA。在核小体核心所包围的DNA之外。这些结果具有重要的影响模型内的染色质纤维的尾部功能，并在体外使用核小体核心颗粒的结构和功能的研究。
Within chromatin, the core histone tail domains play critical roles in regulating the structure and accessibility of nucleosomal DNA within the chromatin fiber. Thus, many nuclear processes are facilitated by concomitant posttranslational modification of these domains. However, elucidation of the mechanisms by which the tails mediate such processes awaits definition of tail interactions within chromatin, In this study we have investigated the primary DNA target of the majority of the tails in mononucleosomes. The results clearly show that the tails bind preferentially to "linker" DNA. outside of the DNA encompassed by the nucleosome core. These results have important implications for models of tail function within the chromatin fiber and for in vitro structural and functional studies using nucleosome core particles.