Altered gene expression levels of matrix metalloproteinases and their inhibitors in periodontitis-affected gingival tissue
Altered gene expression levels of matrix metalloproteinases and their inhibitors in periodontitis-affected gingival tissue
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DOI：
10.1902/jop.2008.070159
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Yoshie, Hiromasa
中科院分区：
文献类型：
--
作者：
Kubota, Takehiko;Itagaki, Manarni;Yoshie, Hiromasa
背景：细胞外基质降解与合成之间的平衡决定了牙周疾病中的牙周附着水平和牙槽骨基质浓度。基质金属蛋白酶（MMPs）已知可降解牙周韧带附着和骨基质蛋白。本研究的目的是检测基质金属蛋白酶及其抑制剂——基质金属蛋白酶组织抑制剂（TIMPs）在牙周炎中不同表达水平的影响。
方法：对16例广泛性慢性牙周炎患者的炎症牙龈组织样本以及14例全身和牙周健康受试者的对照组织样本进行评估。提取总RNA，并通过实时定量逆转录 - 聚合酶链反应测定MMP - 1、 - 3、 - 9、 - 13以及TIMP - 1、 - 2、 - 3、 - 4相对于β - 肌动蛋白的转录水平。
结果：在受牙周炎影响的牙龈组织中，MMP - 1和TIMP - 4的基因转录水平显著更高（P
Background: The balance between the degradation and synthesis of extracellular matrix determines periodontal attachment levels and alveolar bone matrix concentration in periodontal diseases. Matrix metalloproteinases (MMPs) are known to degrade periodontal ligamental attachment and bone matrix proteins. The purpose of this study was to examine the effect of different expression levels of MMPs and their inhibitors, the tissue inhibitors of matrix metalloproteinases (TIMPs), in periodontitis.Methods: Sixteen inflamed gingival tissue samples from subjects with generalized chronic periodontitis and 14 control tissue samples from systemically and periodontally healthy subjects were evaluated. The total RNA was extracted, and the transcript levels for MMP-1, -3, -9, and -13 and TIMP-1, -2, -3, and -4 relative to P-actin were determined by quantitative real-time reverse transcription - polymerase chain reaction.Results: Gene transcript levels for MMP-1 and TIMP-4 were significantly higher in periodontitis-affected gingival tissues (P