Force mapping in epithelial cell migration
Force mapping in epithelial cell migration
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DOI：
10.1073/pnas.0408482102
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发表时间：
2005-02-15
影响因子：
11.1
通讯作者：
Ladoux, B
中科院分区：
文献类型：
--
作者：
du Roure, O;Saez, A;Ladoux, B
我们测量了上皮细胞对基质施加的动态牵引力。力传感器是一个高密度的弹性体微柱阵列，用来支撑细胞。由细胞迁移引起的牵引力是由测量这些柱子的弯曲推断出来的，并与荧光显微镜下的肌动蛋白定位相关。我们使用多饼-粒子跟踪方法来实时估计细胞的机械活动，具有高空间分辨率（低至2微米），由后阵列的周期性施加。在这些实验中，我们使用分化的Madin-Darby犬肾（MDCK）上皮细胞。我们的数据提供了细胞装配所施加的机械力的明确信息。力的最大强度定位在上皮细胞的边缘。肝细胞生长因子促进细胞运动，诱导MDCK细胞强烈的散射活性。因此，我们比较了在肝细胞生长因子治疗后，MDCK细胞在亚融合上皮和分离细胞中产生的力。单层边缘的最大牵引应力对应于比单个细胞测量的值更高的值，可能是由于集体行为。
We measure dynamic traction forces exerted by epithelial cells on a substrate. The force sensor is a high-density array of elastomeric microfabricated pillars that support the cells. Traction forces induced by cell migration are deduced from the measurement of the bending of these pillars and are correlated with actin localization by fluorescence microscopy. We use a multi pie-particle tracking method to estimate the mechanical activity of cells in real time with a high-spatial resolution (down to 2 mum) imposed by the periodicity of the post array. For these experiments, we use differentiated Madin-Darby canine kidney (MDCK) epithelial cells. Our data provide definite information on mechanical forces exerted by a cellular assembly. The maximum intensity of the forces is localized on the edge of the epithelia. Hepatocyte growth factor promotes cell motility and induces strong scattering activity of MDCK cells. Thus, we compare forces generated by MDCK cells in subconfluent epithelia versus isolated cells after hepatocyte growth factor treatment. Maximal-traction stresses at the edge of a monolayer correspond to higher values than those measured for a single cell and may be due to a collective behavior.