Identification of genes transcribed by Pasteurella multocida in rabbit livers through the selective capture of transcribed sequences
Identification of genes transcribed by Pasteurella multocida in rabbit livers through the selective capture of transcribed sequences
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DOI：
10.1111/j.1574-6968.2012.02559.x
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发表时间：
2012-06-01
影响因子：
2.1
通讯作者：
Qu, Liandong
中科院分区：
文献类型：
--
作者：
Guo, Dongchun;Lu, Yan;Qu, Liandong
多杀性巴氏杆菌是一种革兰氏阴性的非运动性球杆菌，是家禽霍乱、牛出血性败血症、猪萎缩性鼻炎和兔鼻塞的病原体。通过选择性捕获转录序列的方法，鉴定了多杀性巴氏杆菌在感染兔肝脏中的差异表达基因谱，并与体外培养的差异表达基因谱进行了比较。共鉴定了31个基因，其中28个编码氨基酸生物合成和代谢、中间代谢和能量代谢的酶，或调节适应性反应、一般微生物应激反应、转运蛋白和分泌蛋白酶的蛋白质。三个是未知的新基因。随机选择代表不同类别的五个基因，并通过实时逆转录-聚合酶链反应分析进行验证。在感染的兔肝中，所有这些都被多杀性巴氏杆菌上调，与体外培养相比，变化范围为1.61- 13.55倍。本研究已经鉴定了与体外生长条件相比，在兔肝脏感染过程中上调的多杀性巴氏杆菌基因。这些基因为进一步研究多杀性巴氏杆菌的致病机制提供了分子基础。
Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera in poultry, hemorrhagic septicemia in cattle, atropic rhinitis in swine, and snuffles in rabbits. The differentially expressed gene profile of P. multocida in infected rabbit livers was identified and compared with that from in vitro culture by selective capture of transcribed sequences. A total of 31 genes were identified, of which 28 encoded enzymes for amino acid biosynthesis and metabolism, intermediary metabolism, and energy metabolism, or proteins for regulatory adaptive responses, general microbial stress response, transport proteins, and secreted proteinases. Three were unknown, novel genes. Five genes representing different categories were chosen randomly and verified by real-time reverse transcriptase-polymerase chain reaction analysis. All were upregulated by P. multocida in infected rabbit livers, with changes ranging from 1.61- to 13.55-fold when compared with in vitro cultures. This study has identified genes of P. multocida that are upregulated during infection of rabbit livers when compared with in vitro growth conditions. The genes will provide a molecular basis for further study of the pathogenesis of P. multocida.