Marsdenia tenacissima extract sensitizes MG63 cells to doxorubicin-induced apoptosis
Marsdenia tenacissima extract sensitizes MG63 cells to doxorubicin-induced apoptosis
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DOI：
10.4238/2014.january.21.3
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发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Qian, H.
中科院分区：
文献类型：
--
作者：
Huang, T.;Gong, W. H.;Qian, H.
通光藤提取物（MTE）是一种新的平板衍生生物技术产品，在化疗领域经常使用，但偶尔有报道。在这项研究中，我们评估了MTE的抗肿瘤活性和相关机制，通过各种生物技术方法。观察MTE和阿霉素单独或联合作用对MG 63骨肉瘤细胞存活率的影响，并观察细胞形态、凋亡率和Fas表达的变化。结果表明，MTE和阿霉素联合应用上调Fas表达，诱导细胞凋亡。50 mg/mLMTE和1 μ g/mL阿霉素联合应用的存活率明显低于单独应用（P < 0.01）。其他生物技术方法也显示了联合施用的诱导效果，其效果比单独施用强得多。以上结果提示，MTE可能通过上调肿瘤细胞Fas的表达而促进阿霉素的化疗效果。
Marsdenia tenacissima extract (MTE) is a new plate-derived biotechnology product that is frequently used, but occasionally reported, in the field of chemotherapy. In this study, we assessed the antitumor activity and related mechanisms of MTE by various biotechnological methods. The survival rates of MG63 osteosarcoma cells treated with MTE and doxorubicin were measured, individually or jointly, and the changes in cellular shape, apoptotic rates, and Fas expression were observed. The results indicated that combination of MTE and doxorubicin up-regulated Fas expression and induced apoptosis. The survival rate of combined application of 50 mg/mL MTE and 1 mu g/mL doxorubicin was significantly lower than that of the individual application (P < 0.01). Other biotechnology methods also showed an apoptosis-inducing effect of combined application that was much stronger than individual application. All of these results suggested that MTE may promote the effects of doxorubicin chemotherapy, perhaps related to the up-regulation of Fas expression in tumor cells.