Label-Free Proteomics of the Fetal Pancreas Identifies Deficits in the Peroxisome in Rats with Intrauterine Growth Restriction
Label-Free Proteomics of the Fetal Pancreas Identifies Deficits in the Peroxisome in Rats with Intrauterine Growth Restriction
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胎儿胰腺的无标记蛋白质组学鉴定了宫内生长受限大鼠的过氧化物酶体缺陷
DOI：
10.1155/2019/1520753
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发表时间：
2019-11-03
影响因子：
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通讯作者：
Liu, Caixia
中科院分区：
文献类型：
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作者：
Liu, Xiaomei;Guo, Yanyan;Liu, Caixia
目的。本研究的目的是鉴定宫内生长受限（IUGR）胎儿胰腺中的差异表达蛋白（DEP），并研究导致 IUGR 成年糖尿病的分子机制。方法。母体蛋白质营养不良诱导IUGR大鼠模型。在胚胎第20天（E20）收集胎儿胰腺。提取、汇集蛋白质，并进行无标记定量蛋白质组分析。进行生物信息学分析（GO 和 IPA）来定义与 DEP 相关的途径和网络。 LC-MS 结果通过蛋白质印迹和/或定量 PCR (q-PCR) 进行确认。测定胎鼠血样中氧化应激-超氧化物歧化酶（Sod）的主要参数。结果。总共鉴定了 57 个 DEP（27 个上调，30 个下调），阈值变化为 1.5 倍，p 值
Aim. The objective of the present study was to identify differentially expressed proteins (DEPs) in the pancreas of a fetus with intrauterine growth restriction (IUGR) and to investigate the molecular mechanisms leading to adulthood diabetes in IUGR. Methods. The IUGR rat model was induced by maternal protein malnutrition. The fetal pancreas was collected at embryonic day 20 (E20). Protein was extracted, pooled, and subjected to label-free quantitative proteomic analysis. Bioinformatics analysis (GO and IPA) was performed to define the pathways and networks associated with DEPs. LC-MS results were confirmed by western blotting and/or quantitative PCR (q-PCR). The principal parameters of oxidative stress-superoxide dismutase (Sod) were determined in blood samples of fetal rats. Results. A total of 57 DEPs (27 upregulated, 30 downregulated) were identified with a 1.5-fold change threshold and a p value