beta-Catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos
beta-Catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos
复制标题
DOI：
10.1016/0925-4773(96)00546-1
复制
发表时间：
1996-07-01
影响因子：
2.6
通讯作者：
Hausen, P
中科院分区：
文献类型：
--
作者：
Schneider, S;Steinbeisser, H;Hausen, P
脊椎动物的背腹两极是如何建立起来的，这一问题对于我们理解脊椎动物的早期发育至关重要。一些证据表明，WNT信号参与了两栖动物Spemann组织者背部特异基因表达的诱导。在这里，我们展示了β-连环蛋白，作为WNT途径的一个组成部分，在非洲爪哇和斑马鱼囊胚的背侧细胞核中瞬时积累。β-连环蛋白的空间限制性核转位先于背部特异基因的表达。在实验室内化的青蛙胚胎中，β-连环蛋白核染色的背腹模式被取消；相反，锂离子或注射Xwnt8-mRNA化的胚胎表现出增强的β-连环蛋白的核积聚。结果表明，在WNT信号之后，β-连环素的转位进入细胞核是蛙和鱼胚胎背腹轴建立的早期步骤。
The question of how dorsal-ventral polarity is established in vertebrates is central to our understanding of their early development. Several lines of evidence suggest that wnt-signaling is involved in the induction of dorsal-specific gene expression in the Spemann Organizer of amphibians. Here, we show that beta-catenin, acting as a component of the wnt-pathway, transiently accumulates in nuclei on the dorsal side of Xenopus and zebrafish blastulae. The spatially restricted nuclear translocation of beta-catenin precedes the expression of dorsal-specific genes. In experimentally ventralized frog embryos the dorsal-ventral pattern of beta-catenin nuclear staining is abolished; in contrast, embryos hyperdorsalized by Li-ions or by injection of Xwnt8-mRNA exhibit an enhanced nuclear accumulation of beta-catenin. The results show that translocation of beta-catenin into nuclei in the wake of wnt-signaling is an early step in the establishment of the dorsal-ventral axis in frog and fish embryos.