Rapid continuous 3D printing of customizable peripheral nerve guidance conduits
Rapid continuous 3D printing of customizable peripheral nerve guidance conduits
复制标题
DOI：
10.1016/j.mattod.2018.04.001
复制
发表时间：
2018-11-01
期刊：
影响因子：
24.2
通讯作者：
Chen, Shaochen
中科院分区：
文献类型：
--
作者：
Zhu, Wei;Tringale, Kathryn R.;Chen, Shaochen
已经证明了工程神经引导导管（NGC）用于修复周围神经损伤。但是，仍然需要先进的生物制作系统来构建具有复杂体系结构，可调材料属性和可自定义的几何控制的NGC。在这里，开发了一个快速连续的3D打印平台，以打印具有前所未有的分辨率，速度，灵活性和可扩展性的可定制NGC。创建了各种复杂性和大小各不相同的NGC设计，其中包括真人大小的仿生分支人的面部NGC。在体内植入小鼠模型的NGC中NGC植入了完全坐骨神经横切，证明了通过分支进入微通道并向损伤部位远端延伸的坐骨神经的有效定向指导。组织学染色和免疫染色进一步证实了整个NGC长度的进行性定向神经再生和分支行为。观察和功能测试，包括冯·弗雷阈值测试和热测试，显示出有望在用3D打印的NGC接枝的同侧肢体中恢复运动功能和感觉。
Engineered nerve guidance conduits (NGCs) have been demonstrated for repairing peripheral nerve injuries. However, there remains a need for an advanced biofabrication system to build NGCs with complex architectures, tunable material properties, and customizable geometrical control. Here, a rapid continuous 3D-printing platform was developed to print customizable NGCs with unprecedented resolution, speed, flexibility, and scalability. A variety of NGC designs varying in complexity and size were created including a life-size biomimetic branched human facial NGC. In vivo implantation of NGCs with microchannels into complete sciatic nerve transections of mouse models demonstrated the effective directional guidance of regenerating sciatic nerves via branching into the microchannels and extending toward the distal end of the injury site. Histological staining and immunostaining further confirmed the progressive directional nerve regeneration and branching behavior across the entire NGC length. Observational and functional tests, including the von Frey threshold test and thermal test, showed promising recovery of motor function and sensation in the ipsilateral limbs grafted with the 3D-printed NGCs.