Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies.
Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies.
复制标题
缺乏基体的莱茵衣藻突变株中有丝分裂纺锤体的空间控制丧失。
DOI：
10.1093/genetics/141.3.945
复制
发表时间：
1995
期刊：
影响因子：
3.3
通讯作者：
Dutcher,SK
中科院分区：
文献类型：
--
作者：
Ehler,LL;Holmes,JA;Dutcher,SK
绿色衣藻中的bld 2 -1突变是唯一已知的导致中心粒/基体丢失以及细胞分裂期间纺锤体位置和卵裂沟位置之间协调丢失的突变。基于几种不同的测定，bld 2 -1细胞在> 99%的细胞中缺乏基体。在bld 2 -1细胞中，在野生型细胞中观察到的定型细胞骨架形态和卵裂沟的精确定位被破坏。有丝分裂纺锤体和卵裂沟的位置彼此之间或与bld 2 -1细胞分裂过程中的特定细胞标志不相关。肌动蛋白在bld 2 -1细胞有丝分裂过程中具有可变的分布，但这种异常分布与纺锤体定位缺陷无关。在野生型和bld 2 -1细胞中，卵裂沟的位置与在绿色藻类中发现的被称为小根微管的一组专门的微管一致。我们建议，根微管执行的功能，星形微管和功能中心粒是必要的组织的细胞骨架的超结构的正确的纺锤体和卵裂沟的位置在衣原体。
The bld2-1 mutation in the green alga Chlamydomonas reinhardtii is the only known mutation that results in the loss of centrioles/basal bodies and the loss of coordination between spindle position and cleavage furrow position during cell division. Based on several different assays, bld2-1 cells lack basal bodies in > 99% of cells. The stereotypical cytoskeletal morphology and precise positioning of the cleavage furrow observed in wild-type cells is disrupted in bld2-1 cells. The positions of the mitotic spindle and of the cleavage furrow are not correlated with respect to each other or with a specific cellular landmark during cell division in bld2-1 cells. Actin has a variable distribution during mitosis in bld2-1 cells, but this aberrant distribution is not correlated with the spindle positioning defect. In both wild-type and bld2-1 cells, the position of the cleavage furrow is coincident with a specialized set of microtubules found in green algae known as the rootlet microtubules. We propose that the rootlet microtubules perform the functions of astral microtubules and that functional centrioles are necessary for the organization of the cytoskeletal superstructure critical for correct spindle and cleavage furrow placement in Chlamydomonas.