Overexpression of MpbHLH transcription factor, an encoding ICE1-like protein, enhances Foc TR4-resistance of Cavendish banana
Overexpression of MpbHLH transcription factor, an encoding ICE1-like protein, enhances Foc TR4-resistance of Cavendish banana
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MpbHLH 转录因子（一种编码 ICE1 样蛋白的蛋白）的过表达可增强卡文迪什香蕉对 Foc TR4 的抗性
DOI：
10.1016/j.scienta.2021.110590
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发表时间：
2021-09-12
影响因子：
4.3
通讯作者：
Dou, Tongxin
中科院分区：
文献类型：
--
作者：
Li, Haochen;Hu, Chunhua;Dou, Tongxin
香蕉(Musa spp.)香蕉是一种重要的热带水果，具有很高的经济价值，但香蕉枯萎病是影响香蕉产业健康发展的关键因素。不同香蕉品种对枯萎病的抗性表现出高度的遗传变异。与卡文迪什香蕉(Musa spp.)AAA组)、大角(Musa spp.ABB组)具有优异的抗病能力，为抗病基因的分离和鉴定提供了优良的测试材料。在本研究中，我们克隆了一个编码ICE1样蛋白转录因子(Tf)的大角候选基因MpbHLH(Basic Helix-loop-Helix)。实时定量聚合酶链式反应(QPCR)结果表明，在低温、盐和脱水条件下，MpbHLH的转录水平被强烈地诱导。在对FocTR4敏感的卡文迪什香蕉中过表达MpbHLH，表明转基因品系对枯萎病的抗性高于野生型(WT)。蛋白质组学分析表明，苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)在转基因植株中得到了高效表达。生理实验进一步表明，转基因植株，特别是其根，具有较高的POD活性。综上所述，MpbHLH在香蕉中的过表达可能会广泛激活与胁迫相关的基因。特别是，POD的活性通过增强转基因植物的ROS清除系统来提高转基因香蕉对枯萎病的抗性。
Banana (Musa spp.) is an important tropical fruit with high economic value, however, Fusarium wilt disease is a critical factor affecting the sound development of the banana industry. Different cultivars of banana exhibit a high degree of genetic variability for resistance to Fusarium wilt. Compared with Cavendish banana (Musa spp. AAA group), Dajiao (Musa spp. ABB group) has superior disease resistance and provides an excellent test material for the isolation and characterization of disease resistance genes. In this study, a Dajiao candidate gene, MpbHLH (basic helix-loop-helix), an encoding ICE1-like protein transcription factor (TF), was cloned and characterized. Real-time quantitative PCR (qPCR) results revealed that MpbHLH transcript levels were strongly induced under cold treatment, salt, and dehydration. Overexpression of MpbHLH in Foc TR4-sensitive Cavendish banana showed that the transgenic lines had superior resistance to Fusarium wilt compared with the wild-type (WT). Proteomic analysis showed that phenylalanine ammonia lyase (PAL) and peroxidase (POD) were highly expressed in transgenic plants. Physiological experiments further revealed that transgenic plants, especially their roots, had high POD activity. Taken together, overexpression of MpbHLH in banana may extensively activate stress-related genes. In particular, the activity of POD increases the resistance of transgenic banana to Fusarium wilt by enhancing the reactive oxygen species (ROS) scavenging system of a transgenic plant.