Purified autoantibodies against glucose-regulated protein 78 (GRP78) promote apoptosis and decrease invasiveness of ovarian cancer cells
Purified autoantibodies against glucose-regulated protein 78 (GRP78) promote apoptosis and decrease invasiveness of ovarian cancer cells
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DOI：
10.1016/j.canlet.2011.05.022
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发表时间：
2011-10-01
期刊：
影响因子：
9.7
通讯作者：
Petignat, Patrick
中科院分区：
文献类型：
--
作者：
Cohen, Marie;Petignat, Patrick
循环葡萄糖调节蛋白78（GRP 78）自身抗体是上皮性卵巢癌的潜在生物标志物。然而，这些抗体在卵巢癌中的识别相对有限。在这里，我们感兴趣的是这些抗体的特征及其在肿瘤发展中的作用。我们首先从卵巢癌患者的血清中纯化了GRP 78自身抗体，然后在SKOV-3卵巢癌细胞系上进行了测试。H2 O2诱导的SKOV-3细胞凋亡增加，侵袭能力降低，提示其对卵巢癌细胞具有保护作用。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Circulating glucose-regulated protein 78 (GRP78) autoantibodies represent potential biomarker of epithelial ovarian cancer. However there is relatively limited identification of these antibodies in response to ovarian cancer. Here, we were interested in characterization of these antibodies and into their role in tumor development. We first purified GRP78 autoantibodies from sera of patients with ovarian cancer, and then tested them on SKOV-3 ovarian cancer cell line. A decrease of invasion and an increase of H2O2-induced apoptosis of SKOV-3 cells were observed, suggesting their protective role against ovarian cancer cells. (C) 2011 Elsevier Ireland Ltd. All rights reserved.