In vitro antiviral activity of 1,2,3,4,6-penta-O-galloyl-β-D-glucose against hepatitis B virus
In vitro antiviral activity of 1,2,3,4,6-penta-O-galloyl-β-D-glucose against hepatitis B virus
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DOI：
10.1248/bpb.29.2131
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发表时间：
2006-10-01
影响因子：
2
通讯作者：
Mar, Woongchon
中科院分区：
文献类型：
--
作者：
Lee, Sung-Jin;Lee, Hak-Kyo;Mar, Woongchon
This study examined the antiviral activity of the root of Paeonia lactiflora PALL. Among the solvent fractions of the crude drug, the ethyl acetate fraction showed anti-hepatitis B virus (HBV) activity (IC50, 8.1 mu g/ml) in an HBV-producing HepG2.2.15 cell culture system. The active anti-HBV principle was isolated and identified as 1,2,3,4,6-penta-O-galloyol-beta-D-glucose (PGG) from the crude drug by activity-guided fractionation. PGG isolated from R lactiflora was examined for the inhibition of HBV multiplication by measurement of HBV DNA and hepatitis B surface antigen (HBsAg) levels in the extracellular medium of HepG2.2.15 cells after 8-d treatment. PGG decreased the level of extracellular HBV (IC50, 1.0 mu g/ml) in a dose-dependent manner. PGG also reduced the HBsAg level by 25% at a concentration of 4 mu g/ml. The gallate structure of PGG may play a critical role in the inhibition of anti-HBV activity. These results suggest that PGG could be a candidate for developing an anti-HBV agent.
