Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (eyene,3/4ae′⟫eEuroae-1) on experimental rabbit model of osteoarthritis
Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (eyene,3/4ae′⟫eEuroae-1) on experimental rabbit model of osteoarthritis
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DOI：
10.1007/s11655-011-0727-x
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发表时间：
2013-06-01
影响因子：
2.9
通讯作者：
Yang Kuo
中科院分区：
文献类型：
--
作者：
Liu Wei;Wu Yuan-hao;Yang Kuo
为观察补肾活血中药加味补肾活血汤（BSHXD，eyene，3/4ae '>> eEuroae-（1））对家兔骨关节炎（OA）模型的代谢、调节和抗氧化作用。将OA模型家兔随机分为5组：模型对照组、阳性对照组、补肾活血汤低、中、高剂量组。另设正常对照组和假手术组。治疗7周后处死实验动物，使用Mankin标准评估软骨组织的病理变化。检测血清和尿液中羟脯氨酸（Hyp）、丙二醛（MDA）含量，血清和膝关节滑膜匀浆中超氧化物歧化酶（SOD）活性和一氧化氮（NO）含量，Mankin评分各治疗组间差异无统计学意义（P> 0.05），但均优于模型对照组（P <0.05）。补肾泻心汤治疗组大鼠血清、尿Hyp、MDA含量及血清、滑膜组织NO含量均显著低于模型组，血清、滑膜组织SOD活性显著高于模型组（P <0.01）;补肾泻心汤的作用呈一定的剂量依赖性，加味补肾泻心汤对实验性骨关节炎兔软骨代谢有改善作用，具有抗过氧化损伤的骨保护作用。
To observe the metabolic, regulatory and anti-oxidative effects of modified Bushen Huoxue Decoction (BSHXD, eyene,3/4ae '>> eEuroae-(1)), a Chinese herbal medicine for Kidney (Shen)-reinforcement and bloodactivation, on an osteoarthritis (OA) rabbit model.A rabbit model for knee joint OA was established by the classic Hulth's method. The OA model rabbits were randomized into 5 groups: the model control group, the positive control group treated with glucosamine sulfate, and the three BSHXD treated groups treated respectively with low, moderate, and high doses of BSHXD. In addition, a normal control group and a sham-operated group were set up. Experimental animals were sacrificed after a 7-week treatment, and pathological changes in cartilaginous tissue were estimated using the Mankin criteria. Hydroxyproline (Hyp) and malonaldehyde (MDA) contents in blood serum and urine, as well as superoxide dismutase (SOD) activity and nitric oxide (NO) content in blood serum and knee joint synovial homogenates were detected.Mankin scoring showed insignificant statistical differences between the various treatment groups (P > 0.05), but all were better than the model control group (P < 0.05). Serum and urinary contents of Hyp and MDA as well as serum and synovial levels of NO were significantly lower, but the SOD activity in blood serum and synovial tissue was higher in the BSHXD treated groups than in the model group P < 0.01); the effect of BSHXD was dose-dependent to some extent.The modified BSHXD shows an effect of improving cartilage metabolism in experimental rabbits with OA, and possesses osteo-chondric protective effects in antagonizing peroxidation injury.