Different domains of the essential GTPase cdc42p required for growth and development of Saccharomyces cerevisiae
Different domains of the essential GTPase cdc42p required for growth and development of Saccharomyces cerevisiae
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DOI：
10.1128/mcb.21.1.235-248.2001
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发表时间：
2001-01-01
影响因子：
5.3
通讯作者：
Braus, GH
中科院分区：
文献类型：
--
作者：
Mösch, HU;Köhler, T;Braus, GH
在芽殖酵母中，Rho型GTdR Cdc 42 p对于细胞分裂是必需的，并且调节假菌丝发育和侵入性生长。在这里，我们分离出新的Cdc 42 p突变体蛋白与单氨基酸取代，足以解偶联功能的Cdc 42 p从所需的假菌丝发育和侵入性生长的调节功能的细胞分裂。在单倍体细胞中，Cdc 42 p能够调节侵袭性生长，依赖于和独立于FLO 11基因的表达。在二倍体细胞中，Cdc 42 p通过控制假菌丝细胞（PH细胞）的形态发生和侵入性生长来调节假菌丝的发育。几个Cdc 42 p突变体分离在这里块PH细胞形态发生响应氮饥饿，而不影响形态或极性的酵母形式的细胞在营养丰富的条件下，表明这些蛋白质受损的某些信号功能。发育特异性Cdc 42 p突变体和已知效应蛋白之间的相互作用研究表明，除了p21激活的（PAK）样蛋白激酶Ste 20 p，Cdc 42 p/Rac相互作用结合结构域包含Gic 1 p和Gic 2 p蛋白和PAK样蛋白激酶Skm 1 p可能是Cdc 42 p的进一步效应物，调节假菌丝和侵入性生长。
In budding yeast, the Rho-type GTPase Cdc42p is essential for cell division and regulates pseudohyphal development and invasive growth. Here, we isolated novel Cdc42p mutant proteins with single-amino-acid substitutions that are sufficient to uncouple functions of Cdc42p essential for cell division from regulatory functions required for pseudohyphal development and invasive growth. In haploid cells, Cdc42p is able to regulate invasive growth dependent on and independent of FLO11 gene expression. In diploid cells, Cdc42p regulates pseudohyphal development by controlling pseudohyphal cell (PH cell) morphogenesis and invasive growth. Several of the Cdc42p mutants isolated here block PH cell morphogenesis in response to nitrogen starvation without affecting morphology or polarity of yeast form cells in nutrient-rich conditions, indicating that these proteins are impaired for certain signaling functions. Interaction studies between development-specific Cdc42p mutants and known effector proteins indicate that in addition to the p21-activated (PAK)-like protein kinase Ste20p, the Cdc42p/Rac-interactive-binding domain containing Gic1p and Gic2p proteins and the PAK-like protein kinase Skm1p might be further effecters Of Cdc42p that regulate pseudohyphal and invasive growth.