Multi-SUMOylation of NAC1 is essential for the growth of prostate cancer cells
Multi-SUMOylation of NAC1 is essential for the growth of prostate cancer cells
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DOI：
10.1016/j.bbrc.2022.12.015
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yingli Wu
中科院分区：
文献类型：
--
作者：
Donghua Wen;Min Hu;Wenzheng Guo;Jingjing Wu;Yingli Wu
ucleus accumbens-associated 1 (NAC1) is a member of pox virus and zinc finger/bric-a-brac tramtrack broad complex (BTB/POZ) gene family. Overexpression of NAC1 is implicated in cancer development, recurrence and chemotherapy resistance. In our previous study, we found NAC1 was a potential small ubiquitin-like modifier (SUMO) substrate in prostate cancer cells. However, there was still lack of evidences to further support and validate the result. In this work, we found that NAC1 is a multi-SUMO-sites acceptor. The SUMO acceptor lysines were K167, K318, K368, K483 and K498. SUMOylation didn't alter the localization of NAC1, but facilitated the formation of NAC1 nuclear bodies. Compared with NAC1 wild type (NAC1 WT), the SUMO-sites mutant of NAC1 (NAC1 SM) suppressed cell proliferation and tumor growth in cellular and animal levels. This work uncovered the function of SUMOylation of NAC1 in prostate cancer cells.
