Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors.
Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors.
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DOI：
10.1007/978-1-4939-7474-0_3
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Allen CDC
中科院分区：
文献类型：
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作者：
Yang Z;Allen CDC
活化后的B细胞可经历其免疫球蛋白基因的类别转换重组和体细胞超突变，并可分化为记忆细胞和浆细胞。在原代B细胞和B细胞系中以改变的形式表达基因是理解B细胞受体信号传导如何导致B细胞活化和分化的重要方法。基于重组逆转录病毒的转导是递送外源基因以在B细胞中表达的最有效的方法。在这一章中，我们描述了使用重组逆转录病毒载体的简化方案，以培养小鼠原代B细胞和B细胞系。
B cells, after activation, can undergo class-switch recombination and somatic hypermutation of their immunoglobulin genes, and can differentiate into memory cells and plasma cells. Expressing genes in altered versions in primary B cells and B cell lines is an important approach to understanding how B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to deliver exogenous genes for expression in B cells. In this chapter, we describe streamlined protocols for using recombinant retroviral vectors to transduce both murine primary B cells and B cell lines.