Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar
Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar
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DOI：
10.1186/s12917-019-2018-2
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发表时间：
2019-07-25
影响因子：
2.6
通讯作者：
Ohashi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Fujisawa, Sotaro;Murata, Shiro;Ohashi, Kazuhiko
背景在包括缅甸在内的东南亚国家，家禽养殖是一项主要产业。为了管理和维持稳定的生产力，必须制定生物安全政策。传染性呼吸道疾病是家禽养殖业的主要威胁。缅甸报告了禽流感和纽卡斯尔病，但没有关于支原体和传染性支气管炎病毒等其他呼吸道病原体的科学资料。确定IBV的基因型和血清型对于告知疫苗接种计划尤其重要。本研究检测了鸡毒支原体（MG）、鸡毒支原体（M. synonymophysis（MS），和IBV在几个家禽农场在Myanmar.ResultsSamples收集了20个农场在缅甸的三个主要家禽养殖区，和MG，MS，和IBV检测到的两个，四个，和八个农场，分别通过聚合酶链反应。系统发育分析显示，观察到的MG和MS分离株与疫苗株不相同。三种不同基因型的IBV被检测到，但没有一个是一个未知的variant.ConclusionsMycoplasmas和IBV在缅甸的家禽养殖场检测。需要定期监测，以确定每种病原体的分布情况，并制定更好的疫苗方案。
BackgroundIn Southeast Asian countries, including Myanmar, poultry farming is a major industry. In order to manage and maintain stable productivity, it is important to establish policies for biosecurity. Infectious respiratory diseases are a major threat to poultry farming. Avian influenza and Newcastle disease have been reported in Myanmar, but no scientific information is available for other respiratory pathogens, such as mycoplasmas and infectious bronchitis virus (IBV). Identifying the genotypes and serotypes of IBVs is especially important to inform vaccination programs. In this study, we detected Mycoplasma gallisepticum (MG), M. synoviae (MS), and IBV in several poultry farms in Myanmar.ResultsSamples were collected from 20 farms in three major poultry farming areas in Myanmar, and MG, MS, and IBV were detected on two, four, and eight farms, respectively, by polymerase chain reaction. Phylogenetic analysis revealed that the observed MG and MS isolates were not identical to vaccine strains. Three different genotypes of IBV were detected, but none was an unknown variant.ConclusionsMycoplasmas and IBV were detected on poultry farms in Myanmar. Periodic surveillance is required to establish the distribution of each pathogen, and to institute better vaccine protocols.