An activation-independent role of transcription factors in insulator function
An activation-independent role of transcription factors in insulator function
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DOI：
10.1093/embo-reports/kve024
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发表时间：
2001-02-01
期刊：
影响因子：
7.7
通讯作者：
Gilson, É
中科院分区：
文献类型：
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作者：
Fourel, G;Boscheron, C;Gilson, É
染色质绝缘体是指转录中性的元件，可以阻止负面或正面的影响穿过染色质延伸到启动子。在这里，我们表明，酵母亚端粒抗沉默区域的行为作为边界端粒驱动的沉默，也允许沉默染色质的不连续繁殖。绝缘子活性的这两个方面，边界和沉默不连续性，可以通过将各种转录激活结构域连接到DNA上的串联位点来概括。重要的是，我们表明，这些绝缘子活动不涉及直接转录激活的报告启动子。这些发现预测，某些启动子的行为作为绝缘体和分区基因组在功能上独立的域。
Chromatin insulators are defined as transcriptionally neutral elements that prevent negative or positive influence from extending across chromatin to a promoter. Here we show that yeast subtelomeric anti-silencing regions behave as boundaries to telomere-driven silencing and also allow discontinuous propagation of silent chromatin. These two facets of insulator activity, boundary and silencing discontinuity, can be recapitulated by tethering various transcription activation domains to tandem sites on DNA. Importantly, we show that these insulator activities do not involve direct transcriptional activation of the reporter promoter. These findings predict that certain promoters behave as insulators and partition genomes in functionally independent domains.