Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells
Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells
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DOI：
10.3892/mmr.2015.4141
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发表时间：
2015-10-01
影响因子：
3.4
通讯作者：
Iida, Junichiro
中科院分区：
文献类型：
--
作者：
Hayakawa, Takako;Yoshimura, Yoshitaka;Iida, Junichiro
正畸力产生的机械应力是牙周膜和牙槽骨改建的一个因素。据报道，当压力作用于成骨细胞和PDL细胞时，与破骨细胞生成相关的许多细胞因子的表达上调。本研究探讨了压力对巨噬细胞系RAW 264.7破骨细胞形成的影响。在培养24 h的第4天，使用3、5、7、9或14层玻璃盖片对破骨细胞施加压缩力。通过计数抗酒石酸酸性磷酸酶染色阳性的细胞数量来确定破骨细胞的数量。破骨细胞生成在第4天和第5天迅速进展。当施加7个滑动力时，具有>8个核的破骨细胞的数量达到峰值，因此认为这是最佳的压缩力。破骨细胞相关基因表达的改变与巨噬细胞在压力下的分化和融合的变化有关;因此，本研究通过逆转录定量聚合酶链反应评估破骨细胞相关基因。破骨细胞相关基因的mRNA表达在最佳压缩3 h后显著增加，而对照组的mRNA表达在24 h后增加。这些结果表明，当施加最佳压缩力时，巨噬细胞的破骨细胞生成加速。
Mechanical stress produced by orthodontic forces is a factor in the remodeling of periodontal ligaments (PDLs) and alveolar bone. It has been reported that the expression of a number of cytokines associated with osteoclastogenesis is upregulated when compressive forces act on osteoblasts and PDL cells. The present study investigated the effects of compressive forces on the formation of osteoclasts from the macrophage cell line RAW264.7. Compressive forces on osteoclasts were exerted using layers of 3, 5, 7, 9 or 14 glass cover slips on the 4th day of culture for 24 h. The number of osteoclasts was determined by counting the number of cells positive for tartrate-resistant acid phosphatase staining. Osteoclastogenesis advanced rapidly on days four and five. The number of osteoclasts with >8 nuclei peaked when the force of 7 slips was applied, which was therefore regarded as the optimal compressive force. Alterations in the expression of osteoclast-associated genes are associated with changes in the differentiation and fusion of macrophages in response to compressive forces; therefore, osteoclast-associated genes were assessed by reverse transcription quantitative polymerase chain reaction in the present study. The mRNA expression of osteoclast-associated genes increased significantly after 3 h of optimal compression, whereas mRNA expression increased after 24 h in the control group. These findings suggested that osteoclastogenesis of macrophages was accelerated when an optimal compressive force was applied.