EXPRESSION AND STRUCTURE OF THE HUMAN NGF RECEPTOR
EXPRESSION AND STRUCTURE OF THE HUMAN NGF RECEPTOR
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DOI：
10.1016/0092-8674(86)90619-7
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发表时间：
1986-11-21
期刊：
影响因子：
64.5
通讯作者：
CHAO, M
中科院分区：
文献类型：
--
作者：
JOHNSON, D;LANAHAN, A;CHAO, M
人神经生长因子（NGF）受体的核苷酸序列已经确定。该3.8kb受体mRNA编码427个氨基酸的蛋白质，其含有28个氨基酸的信号肽、含有4个40个氨基酸的重复序列的胞外结构域（在保守位置处具有6个半胱氨酸残基）、随后是富含丝氨酸/苏氨酸的区域、单个跨膜结构域和155个氨基酸的胞质结构域。神经生长因子受体的细胞外结构域的序列预测一个高度有序的结构，含有一个带负电荷的区域，可以作为配体结合位点。这个结构域在进化过程中是保守的。在小鼠成纤维细胞中转染全长cDNA导致NGF受体的稳定表达，所述NGF受体被针对人NGF受体的单克隆抗体识别并结合[125 I]NGF。
The nucleotide sequence for the human nerve growth factor (NGF) receptor has been determined. The 3.8 kb receptor mRNA encodes a 427 amino acid protein containing a 28 amino acid signal peptide, an extracellular domain containing four 40 amino acid repeats with six cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155 amino acid cytoplasmic domain. The sequence of the extracellular domain of the NGF receptor predicts a highly ordered structure containing a negatively charged region that may serve as the ligand-binding site. This domain is conserved through evolution. Transfection of a full-length cDNA in mouse fibroblasts results in stable expression of NGF receptors that are recognized by monoclonal antibodies to the human NGF receptor and that bind [125I]NGF.