Cell-strain-energy costs of active control of contractility.
Cell-strain-energy costs of active control of contractility.
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主动控制收缩性的细胞应变能量成本。
DOI：
10.1103/physreve.107.l062401
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Solowiej-Wedderburn J
中科院分区：
文献类型：
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作者：
Solowiej-Wedderburn J
细胞机械传感涉及广泛的细胞行为的控制，其中细胞骨架收缩性是关键组成部分。在实验中，观察到细胞的收缩性对基质硬度的增加做出反应，显示出收缩力的增加并改变了细胞骨架元素的分布。在这里，我们使用活跃细胞收缩力的理论模型表明，收缩力的上调不需要耗费大量能量，特别是当与粘附和收缩分布的变化相结合时。事实上，我们表明，基于应变能维持的反馈机制需要上调除最软基材之外的所有基材上的收缩压力。我们考虑常见报道的基底应变能和所做的主动功。我们证明，基底应变能会优先选择实验观察到的较硬基底上的细胞粘附聚集，从而有效地软化基底并实现总收缩压的上调，而收缩性的局部化对内部功的影响最大。
Cell mechanosensing is implicated in the control of a broad range of cell behaviors, with cytoskeletal contractility a key component. Experimentally, it is observed that the contractility of the cell responds to increasing substrate stiffness, showing increased contractile force and changing the distribution of cytoskeletal elements. Here, we show using a theoretical model of active cell contractility that upregulation of contractility need not be energetically expensive, especially when combined with changes in adhesion and contractile distribution. Indeed, we show that a feedback mechanism based on the maintenance of strain energy would require an upregulation in contractile pressure on all but the softest substrates. We consider both the commonly reported substrate strain energy and active work done. We demonstrate substrate strain energy would preferentially select for the experimentally observed clustering of cell adhesions on stiffer substrates which effectively soften the substrate and enable an upregulation of total contractile pressure, while the localization of contractility has the greatest impact on the internal work.