Importin α is an essential nuclear import carrier adaptor required for proper sexual and asexual development and secondary metabolism in Aspergillus nidulans
Importin α is an essential nuclear import carrier adaptor required for proper sexual and asexual development and secondary metabolism in Aspergillus nidulans
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DOI：
10.1016/j.fgb.2009.03.006
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发表时间：
2009-06-01
影响因子：
3
通讯作者：
Espeso, Eduardo A.
中科院分区：
文献类型：
--
作者：
Araujo-Bazan, Lidia;Dhingra, Sourabh;Espeso, Eduardo A.
在真核生物中，主要的核输入途径由输入素α/β 1异二聚体驱动。KapA，构巢曲霉输入素α，是一种必需蛋白。我们产生了一个条件等位基因，kapA 31，模仿酿酒酵母中的srp 1 -31等位基因。KapA 31携带Ser 111 Phe氨基酸取代，其在42 ℃的限制性温度下减少含有经典核定位序列cNLS的货物的核输入。使用kapA 31，我们已经证明了importin α在光依赖性发育调节剂VeA的核积累中的作用。KapA在细胞中有额外的任务，如对Importin alpha家族的其他成员所报道的那样。KapA参与无性和有性发育的不同调节阶段，分别是完成分生孢子和子囊孢子形成的生殖周期所必需的。最后，KapA还介导具有双重作用的次级代谢的不同途径：对青霉素的产生是积极的，对真菌毒素的生物合成是消极的。(C)2009爱思唯尔公司All rights reserved.
In eukaryotes, the principal nuclear import pathway is driven by the importin alpha/beta 1 heterodimer. KapA, the Aspergillus nidulans importin alpha, is an essential protein. We generated a conditional allele, kapA31, mimicking the srp1-31 allele in Saccharomyces cerevisiae. KapA31 carries a Ser111Phe amino acid substitution which, at the restrictive temperature of 42 degrees C, reduces nuclear import of cargos containing classical nuclear-localization-sequences, cNLS. Using kapA31, we have demonstrated the role of the importin alpha in the nuclear accumulation of the light-dependent developmental regulator VeA. KapA have additional tasks in the cell, as reported for other members of the importin alpha family. KapA participates at different regulatory stages of asexual and sexual development, being required for the completion of both reproductive cycles with the formation of conidiospores and ascospores, respectively. Finally, KapA also mediates in different pathways of secondary metabolism having a dual role: positively for penicillin production and negatively for mycotoxin biosynthesis. (C) 2009 Elsevier Inc. All rights reserved.