Sensing a sensor: identifying the mechanosensory function of primary cilia.
Sensing a sensor: identifying the mechanosensory function of primary cilia.
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DOI：
10.3390/bios4010047
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Nauli SM
中科院分区：
文献类型：
--
作者：
Prasad RM;Jin X;Nauli SM
在过去的十年中，初级纤毛已经成为细胞感知和消除机械刺激的主要手段。初级纤毛是感觉细胞器，其已被证明与肾肾单位中的尿液、肝胆系统中的胆汁、胰管中的消化液、牙髓中的牙本质、骨和软骨中的腔隙性管液以及脉管系统中的血液的机械感觉密切相关。哺乳动物细胞类型中初级纤毛的流行与纤毛结构和功能缺陷引起的各种疾病状态相匹配。在描绘这些疾病状态背后的机制的过程中，钙荧光测定法已被广泛用作定量流体流动和药理学试剂的纤毛功能的手段。在这篇综述中，我们将讨论相关的钙水平纤毛功能的方法。
Over the past decade, primary cilia have emerged as the premier means by which cells sense and transduce mechanical stimuli. Primary cilia are sensory organelles that have been shown to be vitally involved in the mechanosensation of urine in the renal nephron, bile in the hepatic biliary system, digestive fluid in the pancreatic duct, dentin in dental pulp, lacunocanalicular fluid in bone and cartilage, and blood in vasculature. The prevalence of primary cilia among mammalian cell types is matched by the tremendously varied disease states caused by both structural and functional defects in cilia. In the process of delineating the mechanisms behind these disease states, calcium fluorimetry has been widely utilized as a means of quantifying ciliary function to both fluid flow and pharmacological agents. In this review, we will discuss the approaches used in associating calcium levels to cilia function.