Metastatic Brain Tumors: Local Blood Flow and Capil- lary Permeability
Metastatic Brain Tumors: Local Blood Flow and Capil- lary Permeability
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转移性脑肿瘤：局部血流和毛细血管通透性
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发表时间：
2006
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通讯作者：
R. Blasberg
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作者：
R. Blasberg
用~4C-碘安替比林(Sakurada等，Am J Physiol234H59,1978)和14C-α-氨基异丁酸(AIB，Blasberg等，神经病学28：363,1978)方法对Walker 256脑转移瘤(Ushio等，Ann Neurol 2：20,1977)大鼠的局部脑血流量和毛细血管通透性进行了研究。取邻近脑组织切片进行放射自显影和组织学染色。与较小的肿块(0.5~0.8ml/gm/min)相比，较大的肿块(0.1~0.2mligndmin)的血流减少更多，并且血流的减少从较大的肿瘤边缘延伸到邻近的皮质(0.4~0.9mvgndmin)。肿瘤对侧皮质血流量为1.3ml/gm/min。在较大的肿瘤肿块内观察到不同的血流，但没有看到与肿瘤的“坏死性”和“活的”细胞之间的边界相对应的明显分界线。肿瘤毛细血管通透性以AIB的血-脑转移率常数表示，在不同肿瘤区域表现出相当大的差异性，是正常脑的2-100倍(2×10-3min-‘)。与坏死区和可变区对应的毛细血管通透性没有明显和一致的差异。肿瘤边缘在组织学上清晰可见。然而，肿瘤边缘和邻近脑组织的通透性梯度变化很大，偶尔会延伸到邻近脑组织几毫米。直径小于1 mm的肿瘤病灶在血流或毛细血管通透性方面没有变化，这表明在这些变化发生之前，肿瘤的临界大小似乎是必要的。
Local cerebral blood flow and capillary permeability measured by the I4C-iodoantipyrine (Sakurada et al, Am J Physiol234H 59, 1978) and 14C-alpha aminoisobutyric acid (AIB) (Blasberg e t al, Neurology 28:363, 1978) methods, was studied in anesthetized rats with Walker 256 metastatic brain tumors (Ushio e t al, Ann Neurol 2:20, 1977). Neighboring brain sections were cut for autoradiography and histologic staining. A greater reduction in blood flow through the larger tumor masses (0.1 to 0.2 mligndmin) in comparison to the smaller lesions (0.5 to 0.8 ml/gm/ min) was observed, and the reduction in flow extended beyond the larger tumor margins into neighboring cortex (0.4 to 0.9 mvgndmin). Cortical blood flow contralateral to the tumors was 1.3 ml/gm/min. Differences in flow within the larger tumor masses were observed but a sharp demarcation corresponding to the border between "necrotic" and "viable" cells of the tumor was not seen. The permeability oftumor capillaries, expressed as a blood-to-brain transfer rate constant for AIB, demonstrated considerable variability within different tumor regions and ranged from 2 to 100 times as high as that of normal brain (2 x 10-3 min-'). A clear and consistent difference in capillary permeability corresponding to necrotic and variable tumor regions was not seen. The tumor margins were sharply defined histologically. However, the permeability gradient a t the tumor margin and adjacent brain tissue varied and occasionally extended several millimeters into adjacent brain. Tumor foci smaller than 1 mm in diameter demonstrated no changes in either blood flow or capillary permeability, suggesting that a critical tumor size appears to be necessary before these changes occur.