Stage- and cell-specific expression of cyclic adenosine 3',5'-monophosphate-dependent protein kinases in rat seminiferous epithelium.
Stage- and cell-specific expression of cyclic adenosine 3',5'-monophosphate-dependent protein kinases in rat seminiferous epithelium.
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大鼠生精上皮中环腺苷 3,5-单磷酸依赖性蛋白激酶的阶段和细胞特异性表达。
DOI：
10.1095/biolreprod46.6.1057
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发表时间：
1992
影响因子：
3.6
通讯作者：
H. Persson
中科院分区：
文献类型：
--
作者：
P. Lönnerberg;M. Parvinen;T. Jahnsen;V. Hansson;H. Persson
研究了编码环 AMP (cAMP) 依赖性蛋白激酶 (PKAs) 的大鼠睾丸中 mRNA 的表达。结合Northern印迹和原位杂交，采用显微解剖方法分离代表生精上皮周期不同阶段的10组生精小管。结果显示调节亚基 (PKA-R) 的四种亚型在周期的不同阶段存在差异表达。在所有阶段检测到的 RI α mRNA 水平大致相同，而 RI β mRNA 的表达在 XIII-III 阶段较低，在 IV-V 阶段开始增加，并在 VIII-XI 阶段达到最大值。 RII α mRNA 水平在 II-VI 期较低，在 VIIa、b 期显着升高，在 VIIc、d 和 VIII 期达到最高水平，随后表达降低，RII β mRNA 水平在 VI 期显着升高，在 VII 和 VIII 期达到最高水平。对成年大鼠睾丸切片的原位杂交显示，粗线期精母细胞层和各个阶段的圆形精子细胞中都有 RI α mRNA。在粗线期晚期精母细胞和 VII-XIII 期的圆形精子细胞中检测到 RI beta mRNA。 RII α mRNA 在 VII-VIII 期的圆形精子细胞层和后期的伸长精子细胞中可见，而 RII β mRNA 仅在 VII-VIII 期的圆形精子细胞区域和 I-VI 期的一些小管中检测到。这些数据表明，编码 PKA-R 的 mRNA 在分化的雄性生殖细胞中以阶段特异性的方式表达，每个亚基具有不同的表达模式；这表明这些蛋白激酶在精子发生的不同时期具有特定的作用。
Expression of mRNAs in the rat testis encoding cyclic AMP (cAMP)-dependent protein kinases (PKAs) was studied. A microdissection method was used to isolate 10 pools of seminiferous tubules representing various stages of the cycle of the seminiferous epithelium in combination with Northern blots and in situ hybridization. The results showed a differential expression of the four isoforms of the regulatory subunits (PKA-R) at various stages of the cycle. RI alpha mRNA was detected at approximately the same levels at all stages while expression of RI beta mRNA was low at stages XIII-III, started to increase at stages IV-V, and reached a maximum at stages VIII-XI. The level of RII alpha mRNA was low at stages II-VI, increased markedly at stage VIIa,b, and reached maximal levels at stages VIIc,d and VIII, followed by a reduced expression at later stages, RII beta mRNA levels increased significantly at stage VI with maximal levels at stages VII and VIII. In situ hybridization of sections from the adult rat testis revealed RI alpha mRNA in the layers of pachytene spermatocytes and round spermatids of all stages. RI beta mRNA was detected over late pachytene spermatocytes and round spermatids of stages VII-XIII. RII alpha mRNA was seen in the layers of round spermatids of stages VII-VIII and elongating spermatids of later stages while RII beta mRNA was detected only in the round spermatid region of stages VII-VIII and in some tubules of stages I-VI. These data show that mRNAs encoding PKA-R are expressed in a stage-specific manner in differentiating male germ cells with different patterns of expression for each subunit; this suggests specific roles for these protein kinases at different times of spermatogenesis.