Mapping and initiation studies on the leader RNA of vesicular stomatitis virus.
Mapping and initiation studies on the leader RNA of vesicular stomatitis virus.
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水泡性口炎病毒前导RNA的定位和启动研究。
DOI：
10.1016/0042-6822(77)90423-8
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发表时间：
1977
期刊：
影响因子：
3.7
通讯作者：
A. Banerjee
中科院分区：
文献类型：
--
作者：
R. Colonno;A. Banerjee
水泡性口炎病毒除了在体外合成5种单顺反子外，还合成一个前导RNA。前导RNA分子似乎是一个启动的RNA分子，因为它在5‘端被多聚磷酸化。此外，对纯化的病毒粒子的紫外线灭活研究表明，先导RNA是在mRNA合成之前合成的。前导RNA编码在基因组RNA的3‘端，因为纯化的前导RNA将保护基因组RNA的3’末端区域免受核糖核酸酶的消化。动力学研究表明，42 S基因组RNA中编码N蛋白的前导核糖核酸与编码N蛋白的核糖核酸以相同的速率退变，表明42 S基因组核糖核酸中的前导核糖核酸只编码一次。在没有UTP的情况下，在体外合成一个含有与前导RNA相同的5‘末端序列的寡核苷酸。这些结果表明，前导RNA的合成是水泡性口炎病毒体外RNA转录过程中的第一个事件。
Vesicular stomatitis virus synthesizes a leader RNA in addition to the five monocistronic mRNA speciesin vitro. The leader RNA molecule appears to be an initiated RNA molecule since it is polyphosphorylated at its 5′-terminus. In addition, uv inactivation studies on purified virions suggest that leader RNA is synthesized prior to mRNA synthesis. The leader RNA is coded for at the 3′-end of genome RNA since purified leader RNA will protect the 3′-terminal region of the genome RNA from RNase digestion. Kinetic studies show that leader RNA and mRNA coding for the N protein anneal to 42 S genome RNA at the same rate, indicating that the leader RNA is coded for only once on the 42 S genome RNA. In the absence of UTP, an oligonucleotide is synthesizedin vitrowhich contains the same 5′-terminal sequence as the leader RNA. These results indicate that the synthesis of the leader RNA is the first event duringin vitroRNA transcription by vesicular stomatitis virus.