Gene expression profiling of reproductive meristem types in early rice inflorescences by laser microdissection
Gene expression profiling of reproductive meristem types in early rice inflorescences by laser microdissection
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DOI：
10.1111/tpj.13147
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发表时间：
2016-04-01
期刊：
影响因子：
7.2
通讯作者：
Kater, Martin M.
中科院分区：
文献类型：
--
作者：
Harrop, Thomas W. R.;Din, Israr Ud;Kater, Martin M.
在水稻中，花序结构在生殖发育的早期阶段建立，并直接有助于谷物产量潜力。在诱导开花后，分枝的复杂性以及因此穗上种子的数量由不同分生组织类型的活性和它们之间的过渡时间决定。虽然参与这些转换的一些基因已经被确定，但对控制这一过程的转录调节因子网络的理解仍然缺乏。为了解决这个问题，我们使用了精确的激光显微切割和RNA测序的方法在水稻ssp。日本Nipponbare的目的是产生定量数据，描述四种不同分生组织类型中基因表达的景观：轴分生组织、初生分支分生组织、伸长的初生分支分生组织（包括腋生分生组织）和小穗分生组织。观察到顶端和腋生分生组织类型之间的表达谱转换，随后在腋生分生组织身份转换期间发生更渐进的变化，并确定了几个可能参与分支的基因。这种资源将是至关重要的花序发育和粮食产量之间的联系的机械理解。
In rice, inflorescence architecture is established at early stages of reproductive development and contributes directly to grain yield potential. After induction of flowering, the complexity of branching, and therefore the number of seeds on the panicle, is determined by the activity of different meristem types and the timing of transitions between them. Although some of the genes involved in these transitions have been identified, an understanding of the network of transcriptional regulators controlling this process is lacking. To address this we used a precise laser microdissection and RNA-sequencing approach in Oryza sativa ssp. japonica cv. Nipponbare to produce quantitative data that describe the landscape of gene expression in four different meristem types: the rachis meristem, the primary branch meristem, the elongating primary branch meristem (including axillary meristems), and the spikelet meristem. A switch in expression profile between apical and axillary meristem types followed by more gradual changes during transitions in axillary meristem identity was observed, and several genes potentially involved in branching were identified. This resource will be vital for a mechanistic understanding of the link between inflorescence development and grain yield.