Eosinophil influx into the airways in patients with exercise-induced asthma
Eosinophil influx into the airways in patients with exercise-induced asthma
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DOI：
10.1053/rmed.2000.0951
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发表时间：
2000-12-01
影响因子：
4.3
通讯作者：
Fireman, E
中科院分区：
文献类型：
--
作者：
Kivity, S;Argaman, A;Fireman, E
哮喘是一种常见的疾病，其发病机制尚未完全阐明。嗜酸性粒细胞被认为在其发生中起作用。为研究运动性哮喘对自发痰细胞和介质组成的影响，对25例支气管哮喘患者自发痰进行了研究。一组（n=9）和一个没有（n=9）运动诱发哮喘进行运动挑战。第三组（n=7）进行乙酰甲胆碱激发。使用Giemsa染色法分析痰液的细胞分类计数，测量嗜酸性粒细胞阳离子蛋白和白三烯混合物运动性哮喘组FEV 1平均下降23.7 ± 7.4%，显著低于对照组（P < 0.05）。（P=0.001）高于未使用组。痰嗜酸性粒细胞仅在运动性哮喘组中显著增加（从8.1+/-13.9%至18.3+/-20.2%，P=0.0017），而对照组（从0.9+/-0.9%至1.5+/-1.5%）或乙酰甲胆碱激发组（从23.6+/-27.2%至22.3+/-23.8%）则没有。嗜酸性粒细胞阳离子蛋白在任何组中均无显著变化。在痰液中，有6或7名运动诱发哮喘患者的白三烯含量在运动后增加，嗜酸性粒细胞流入运动诱发哮喘患者的气道可以部分解释这些患者气道中的白三烯。
Exercise-induced asthma is a common phenomenon, the mechanism of which is undetermined. Eosinophils have been suggested as playing a role in its occurrence. We studied the effect of exercise-induced asthma on the cellular and mediator composition of spontaneously obtained sputum.Twenty-five patients with bronchial asthma were investigated by studying sputum spontaneously obtained before and following challenge. One group with (n=9) and one without (n=9) exercise-induced asthma performed exercise challenge. A third group (n=7) performed methacholine challenge. The sputum was analysed using Giemsa staining for differential cell count, measuring eosinophil cationic proteins and mixtures of leukotrienes (D4, E4 and C4) in the liquid phase using ELISA.The group with exercise-induced asthma had a mean drop of 23.7+/-7.4% in FEV1, significantly (P=0.001) higher than the group without it. Following challenges, there were significant increases in sputum eosinophils only in the group with exercise-induced asthma (from 8.1+/-13.9% to 18.3+/-20.2%, P=0.0017) and not in control groups (from 0.9+/-0.9% to 1.5+/-1.5%) or in those who had methacholine challenge (from 23.6+/-27.2% to 22.3+/-23.8%). Eosinophil cationic proteins did not change significantly in any group. In the liquid phase of the sputum, the amount of leukotrienes increased following exercise in six or the seven patients with exercise-induced asthma in whom it was measured.The influx of eosinophils to the airway in patients who develop exercise-induced asthma can be partially explained by the leukotrienes in the airways of those patients.