The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea
The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea
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DOI：
10.1007/s10265-010-0369-7
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发表时间：
2011-03-01
影响因子：
2.8
通讯作者：
Iida, Shigeru
中科院分区：
文献类型：
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作者：
Johzuka-Hisatomi, Yasuyo;Noguchi, Hiroshi;Iida, Shigeru
常见牵牛花 (Ipomoea purpurea) 的 A 基因座上的可变 a(flaked) (a(f)) 等位基因在花色素沉着中赋予不完全优势，是由 DNA 转座子 Tip100 插入 CHS-D 引起的，CHS-D 编码查尔酮合酶，是花青素生物合成所需的。杂合花中 CHS-D 转录物、CHS-D 蛋白和花青素色素的水平约为纯合花的一半，表明 CHS-D 的剂量依赖性表达是观察到的不完全优势的主要原因。这与金鱼草 (Antirhinum majus) 中的妮维雅基因座形成对比，其中不完全显性是由半显性 CHS 等位基因引起的。
The mutable a(flaked) (a(f)) allele at the A locus of the common morning glory (Ipomoea purpurea) confers incomplete dominance in flower pigmentation and is caused by insertion of the DNA transposon Tip100 into CHS-D, which encodes chalcone synthase and is required for anthocyanin biosynthesis. Levels of CHS-D transcripts, CHS-D protein, and anthocyanin pigment in heterozygous flowers were about half that in homozygous flowers, indicating that dosage-dependent expression of CHS-D is the primary cause of the observed incomplete dominance. This contrasts with the Nivea locus in snapdragon (Antirrhinum majus) in which incomplete dominance is caused by semidominant CHS alleles.