Analysis of sugars and amino acids in aphid honeydew by hydrophilic interaction liquid chromatography – Mass spectrometry
Analysis of sugars and amino acids in aphid honeydew by hydrophilic interaction liquid chromatography – Mass spectrometry
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DOI：
10.1016/j.mex.2020.101050
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发表时间：
2020-09
期刊：
影响因子：
1.9
通讯作者：
Phuong K. Nguyen;J. Owens;Luis E. Lowe;E. Mooney
中科院分区：
文献类型：
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作者：
Phuong K. Nguyen;J. Owens;Luis E. Lowe;E. Mooney
过去的分析蚜虫蜜瓜的糖和氨基酸组成已完成使用不同的仪器。在这里，我们报告了使用亲水相互作用液相色谱（HILIC）耦合三重四极杆质谱（MS/MS）检测器的分析7个木糖（木糖，果糖，葡萄糖，蔗糖，海藻糖，三聚糖和棉子糖）和5个氨基酸（谷氨酸，谷氨酰胺，天冬氨酸，丝氨酸和天冬酰胺）。糖的定量限范围为0.05 mg/L（三糖）至1.0 mg/L（果糖），氨基酸的定量限范围为0.10 mg/L（谷氨酸）至3.66 mg/L（天冬酰胺）。样品制备快速而简单，仅需要用热水（80 °C）洗涤用于收集蚜虫蜜露的箔，并在对两类分析物进行HILIC/MS/MS分析之前对样品进行超声处理。不需要分析物衍生化，观察到优异的色谱特性。对于那些在该领域研究蜜瓜介导的相互作用的人来说，这项技术可以快速表征具有生态重要性的氨基酸和糖。·采用亲水作用液相色谱-质谱联用技术分析了七种蚜虫的木糖、果糖、葡萄糖、蔗糖、海藻糖、三糖和棉子糖以及五种标准氨基酸谷氨酸、谷氨酰胺、天冬氨酸、丝氨酸和天冬酰胺的组成。使用HILIC-MS分析所有极性分析物，无需衍生化，色谱运行时间为7 min（糖）和10 min（氨基酸）。
Past analyses of sugar and amino acid composition of aphid honeydews have been completed using diverse instrumentation. Here we report the use of hydrophilic interaction liquid chromatography (HILIC) coupled with a triple quadrupole mass spectrometric (MS/MS) detector for the analysis of seven saccharides (xylose, fructose, glucose, sucrose, trehalose, melezitose and raffinose) and five amino acids (glutamic acid, glutamine, aspartic acid, serine, and asparagine). Limits of quantitation ranged from 0.05 mg/L (melezitose) to 1.0 mg/L (fructose) for sugars and from 0.10 mg/L (glutamic acid) to 3.66 mg/L (asparagine) for amino acids. Sample preparation was fast and simple, requiring only the washing of foils used to collect aphid honeydew with hot (80 °C) water and sonication of samples prior to HILIC/MS/MS analysis for both classes of analytes. No analyte derivatization was required and excellent chromatographic characteristics were observed. For those studying honeydew-mediated interactions in the field, this technique allows for rapid characterization of ecologically important amino acids and sugars.•Composition of seven saccharides inAphis asclepiadishoneydew including xylose, fructose, glucose, sucrose, trehalose, melezitose,and raffinose, and five standard amino acids including glutamic acid, glutamine, aspartic acid, serine, and asparagine, were analyzed using hydrophilic interaction liquid chromatography-mass spectrometry.•All polar analytes were analyzed without derivatization using HILIC-MS with chromatographic run times of 7 min (sugars) and 10 min (amino acids).