Plasmonic Interferometer Array Biochip as a New Mobile Medical Device for Cancer Detection.
Plasmonic Interferometer Array Biochip as a New Mobile Medical Device for Cancer Detection.
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等离子体干涉仪阵列生物芯片作为一种用于癌症检测的新型移动医疗设备。
DOI：
10.1109/jstqe.2018.2865418
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Gan,Qiaoqiang
中科院分区：
文献类型：
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作者：
Zeng,Xie;Yang,Yunchen;Zhang,Nan;Ji,Dengxin;Gu,Xiaodong;Jornet,Josep;Wu,Yun;Gan,Qiaoqiang
我们报告了一种等离子体干涉仪阵列（PIA）传感器，并证明了它能够以高灵敏度和低成本实时检测循环外泌体蛋白，从而能够早期检测癌症。具体地，由纳米槽环发射的表面等离子体波在中心纳米孔的输出处与自由空间光干涉，并导致强度干涉图案。在单波长照明下，当靶外泌体蛋白被结合在表面上的抗体捕获时，中心孔和槽环之间的折射率的生物介导的变化引起透射光的强度变化。通过实时记录强度变化，可以有效地筛选生物分子结合事件并分析结合动力学。通过对多对传感器信号进行积分，提高信噪比，实现了折射率变化为1.63 × 10- 6折射率单位、外泌体检测为3.86 × 10- 8/mL的上级传感分辨率。重要的是，这种PIA传感器可以通过与智能手机耦合的小型化显微镜系统成像，以实现便携式和高灵敏度的医疗设备。使用基于商用智能手机的便携式传感系统，实现了9.72 × 109外泌体/mL的外泌体检测传感分辨率。
We report a plasmonic interferometer array (PIA) sensor and demonstrate its ability to detect circulating exosomal proteins in real-time with high sensitivity and low cost to enable the early detection of cancer. Specifically, a surface plasmon wave launched by the nano-groove rings interferes with the free-space light at the output of central nano-aperture and results in an intensity interference pattern. Under the single-wavelength illumination, when the target exosomal proteins are captured by antibodies bound on the surface, the biomediated change in the refractive index between the central aperture and groove rings causes the intensity change in a transmitted light. By recording the intensity changes in real-time, one can effectively screen biomolecular binding events and analyze the binding kinetics. By integrating signals from multiple sensor pairs to enhance the signal-to-noise ratio, the superior sensing resolutions of 1.63 × 10-6refractive index unit in a refractive index change and 3.86 × 10-8exosomes/mL in exosome detection were realized, respectively. Importantly, this PIA sensor can be imaged by a miniaturized microscope system coupled with a smartphone to realize a portable and highly sensitive healthcare device. The sensing resolution of 9.72 × 109exosomes/mL in exosome detection was realized using the portable sensing system building upon a commercial smartphone.