Escherichia coli chromosome-based T7-dependent constitutive overexpression system and its application to generating a phenylalanine producing strain
Escherichia coli chromosome-based T7-dependent constitutive overexpression system and its application to generating a phenylalanine producing strain
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DOI：
10.1016/j.jbiosc.2018.05.014
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发表时间：
2018-11-01
影响因子：
2.8
通讯作者：
Ohmoto, Takashi
中科院分区：
文献类型：
--
作者：
Koma, Daisuke;Kishida, Takahiro;Ohmoto, Takashi
已经尝试了许多代谢工程方法来产生能够产生有价值的化合物的菌株。主要目标之一是这些菌株的工业应用。将合成途径基因整合到大肠杆菌染色体中能够产生稳定且可用于工业应用的无质粒菌株。不需要诱导的菌株在成本方面是有利的。在本研究中，我们在大肠杆菌中构建了一个组成型过表达系统。大肠杆菌，以产生无质粒和无诱导物的菌株。17 RNA聚合酶/T7启动子过表达系统是一种异丙基-β-D-硫代半乳糖苷（IPTG）诱导的基因过表达系统（T7依赖的诱导型过表达系统），经改造后成为组成型过表达系统。将构建的“基于染色体的T7依赖型组成型过表达系统”应用于代谢工程研究，获得了一株无质粒、无诱导剂的苯丙氨酸产生菌。杆菌(C)2018年，日本生物技术学会。All rights reserved.
Many metabolic engineering approaches have been attempted to generate strains capable of producing valuable compounds. One of main goals is industrial application of these strains. Integration of synthetic pathway genes into the Escherichia coli chromosome enables generation of a plasmid-free strain that is stable and useful for industrial applications. Strains that do not require induction are advantageous in terms of cost. In the present study, we constructed a constitutive overexpression system in E. coli to generate plasmid-free and inducer-free strains. The 17 RNA polymerase/T7 promoter overexpression system, which is an isopropyl- beta-D-thiogalactopyranoside (IPTG)-inducible gene over expression system (T7-dependent inducible overexpression system), was modified to be a constitutive overexpression system. The constructed overexpression system, a "chromosome-based T7-dependent constitutive overexpression system", was applied in a metabolic engineering study to generate a plasmid-free and inducer-free phenylalanine producing strain of E. coli. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.