Grass carp reovirus capsid protein interacts with cellular proteasome subunit beta-type 7: Evidence for the involvement of host proteasome during aquareovirus infection
Grass carp reovirus capsid protein interacts with cellular proteasome subunit beta-type 7: Evidence for the involvement of host proteasome during aquareovirus infection
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草鱼呼肠孤病毒衣壳蛋白与细胞蛋白酶体亚基β-7型相互作用：水呼肠孤病毒感染过程中宿主蛋白酶体参与的证据
DOI：
10.1016/j.fsi.2019.12.047
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发表时间：
2020
影响因子：
4.7
通讯作者：
Lu Liqun
中科院分区：
文献类型：
--
作者：
Wang Longlong;Yu Fei;Xu Ning;Lu Liqun
真核蛋白酶体是一种大型的多亚基复合物，通过降解不需要或受损的蛋白质在广泛的基本细胞功能中发挥重要作用，也可以被病毒逆转或操纵以促进病毒感染。在这项研究中，我们证明了蛋白酶体亚单位β - 7型（PSMB7）是一种蛋白酶体组成蛋白，对蛋白酶体的组装很重要，可以与草鱼呼肠病毒（GCRV）衣壳蛋白相互作用。酵母2杂交实验表明，基因型ⅢGCRV的衣壳蛋白VP38可以与PSMB7结合，并通过下拉、共免疫沉淀和亚细胞共定位实验进一步证实了这种相互作用。此外，VP38同源蛋白，基因I型的VP7和基因II型的VP35也可以在类似的蛋白相互作用实验中与宿主PSMB7相互作用。最后，PSMB7基因的表达水平在GCRV感染期间保持稳定，而PSMB7基因的转录在GCRV攻击时受到抑制；在病毒感染期间，与PSMB7的相互作用不会导致VP7或VP38的蛋白降解。因此，宿主PSMB7与病毒衣壳蛋白之间的相互作用可能表明，干扰PSMB7介导的蛋白酶体组装可能与有效的水病毒感染有关。
The eukaryotic proteasome is a large multi-subunit complex that plays an important role in a wide range of fundamental cellular functions by degrading un-needed or damaged proteins, which also can be inverted or manipulated by viruses to favor viral infection. In this study, we demonstrated that proteasome subunit beta-type 7 (PSMB7), a proteasome-constitutive protein that is important for proteasome assembly, interacts with grass carp reovirus (GCRV) capsid proteins. Yeast 2-hybrid assay indicates that capsid protein VP38 of genotype Ⅲ GCRV could bind PSMB7, and this mutual interaction was further confirmed by pull-down, co-immunoprecipitation and subcellular co-localization assays. Furthermore, VP38 homologous proteins, VP7 from genotype I and VP35 from genotype II GCRV, can also interact with host PSMB7 in similar protein-protein interaction assays. Finally, PSMB7 expression level remains stable during GCRV infection, while,psmb7gene transcription was repressed upon GCRV challenge; interaction with PSMB7 doesn't result in protein degradation of either VP7 or VP38 during viral infection. Thus, the interaction between host PSMB7 and viral capsid protein might suggest that interfering with PSMB7-mediated proteasome assembly should be involved in efficient aquareovirus infection.