[Expressions and significance of cyclinD1 in epithelial ovarian cancer cell 3AO].
[Expressions and significance of cyclinD1 in epithelial ovarian cancer cell 3AO].
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DOI：
10.3760/cma.j.issn.0376-2491.2012.05.017
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发表时间：
2012-02
影响因子：
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通讯作者：
J. Dai;Pei Zhang;Peishu Liu;H. Qu
中科院分区：
文献类型：
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作者：
J. Dai;Pei Zhang;Peishu Liu;H. Qu
目的探讨卵巢癌细胞3AO中cyclinD 1的表达及其与卵巢癌细胞增殖的关系。方法体外培养人卵巢上皮细胞和卵巢癌细胞3AO。采用逆转录-聚合酶链反应（RT-PCR）和流式细胞术检测顺铂激活3AO细胞前后CyclinD 1基因和蛋白的表达。观察细胞活性和细胞周期。结果卵巢癌细胞中cyclinD 1基因扩增异常，cyclinD 1过表达，而正常卵巢上皮细胞中cyclinD 1表达阴性。RT-PCR结果显示，顺铂作用下3AO细胞cyclinD 1基因表达有差异。顺铂浓度越高，cyclinD 1基因表达越低。流式细胞仪检测结果显示，顺铂作用下3AO细胞cyclinD 1蛋白表达较未作用时明显降低，且随着顺铂浓度的升高，cyclinD 1蛋白表达逐渐降低。细胞凋亡率分别为0.63%、9.08%和27.41%，增殖指数（PI）分别为38.83%、44.54%和37.31%。差异有统计学意义（P < 0.01）。结论卵巢癌细胞中cyclinD 1表达上调。cyclinD 1的低表达与顺铂浓度呈正相关。cyclinD 1的表达与卵巢癌细胞活性密切相关。
OBJECTIVE To explore the expression of cyclinD1 in ovarian carcinoma cell 3AO and analyze its relationship with the proliferation of ovarian cancer cell. METHODS Human ovarian epithelium and ovarian cancer cells 3AO were cultured in vitro. CyclinD1 genes and proteins were detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry before and after the activation of cell 3AO by cis-platinum. Also the cell activity and cell cycle were observed. RESULTS Abnormal gene amplification and over-expressions of cyclinD1 were found in ovarian cancer cell while the expression of cyclinD1 was negative in normal ovarian epithelium. Under cis-platinum, different expressions of cyclinD1 genes were found in 3AO by RT-PCR. The higher the concentrations of cisplatin, the lower expressions of cyclinD1 genes. By flow cytometry, it was also found that there were lower expressions of cyclinD1 protein in 3AO under cisplatin than without it. With the rising concentrations of cisplatin, the low expressions of cyclinD1 protein in 3AO were detected. The mean numbers were 105.9, 15.42 and 8.59, the cell apoptotic rates 0.63%, 9.08% and 27.41% and the proliferation index (PI) numbers 38.83%, 44.54%, 37.31%. The differences were statistically significant (P < 0.01). CONCLUSION The up-regulation of cyclinD1 is detected in ovarian cancer cell. A positive correlation is found between the lower expressions of cyclinD1 and the concentration of cisplatin. There is a close relationship between the expressions of cyclinD1 and ovarian cancer cell activity.