Binding of cationic cell-permeable peptides to plastic and glass
Binding of cationic cell-permeable peptides to plastic and glass
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DOI：
10.1016/s0196-9781(02)00270-x
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发表时间：
2003-01-01
期刊：
影响因子：
3
通讯作者：
Miller, BT
中科院分区：
文献类型：
--
作者：
Chico, DE;Given, RL;Miller, BT
来源于同源异型蛋白Antp或转录调节因子达特的亲水区域的细胞穿透肽已被用于将几种肽和寡核苷酸货物转运到细胞内部。这种载体肽部分地穿透细胞，因为它们含有多个赖氨酸和精氨酸残基。使用共价连接到Antp或Tat相关载体或D-Arg七聚体的放射性标记的肽货物，我们发现大量的标签仍然紧密结合在塑料和玻璃表面。标记的缀合物的结合完全是由于阳离子载体部分。在某些条件下，这种非特异性结合可能被误认为是细胞渗透。(C)2002年爱思唯尔科学公司All rights reserved.
Cell-penetrating peptides derived from hydrophilic regions of the homeoprotein Antennapedia (Antp) or the transcription-regulating factor Tat have been used to transport several peptide and oligonucleotide cargoes into the interior of cells. Such vector peptides penetrate cells, in part, because they contain multiple lysine and arginine residues. Using radiolabeled peptide cargoes covalently linked to Antp- or Tat-related vectors, or to D-Arg heptamers, we found that a significant amount of the label remained tightly bound to plastic and glass surfaces. Binding of the labeled conjugates was due entirely to the cationic vector moieties. Under certain conditions, such non-specific binding could be mistaken for cellular penetration. (C) 2002 Elsevier Science Inc. All rights reserved.